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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,850 results for: nin

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Showing: 281 - 300 of 66850 results

  2. MoClo Yeast Toolkit

    Plasmid kit
    #1000000061
    Purpose
    Collection of standardized parts for hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression.
    Depositor
    John Dueber
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139999-plasmid-map-sequence-id-346119

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  9. xCas9(3.7)-BE4

    Plasmid
    #108381
    Purpose
    Mammalian expression vector for xCas9(3.7)-BE4
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.7)-BE4
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108381-plasmid-map-sequence-id-217618

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  10. eeBxb1

    Plasmid
    #222339
    Purpose
    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells
    Depositor
    David Liu
    Publication
    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon-optimized evolved and engineered Bxb1
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    222339-plasmid-map-sequence-id-473210

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  11. MoClo Tool Kit

    Plasmid kit
    #1000000044
    Purpose
    Collection of standardized genetic modules that can be used for hierarchical assembly of multi-gene expression constructs.
    Depositor
    Sylvestre Marillonnet
    Application
    Cloning and Synthetic Biology
    Vector type
    Eukaryotic Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    April 8, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  15. pDest-565

    Plasmid
    #11520
    Purpose
    Gateway destination vector for bacterial expression; His,GST tag
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway Cloning
    Tags
    6xHis and GST
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    11520-plasmid-map-sequence-id-324017

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  17. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

    Plasmid
    #124428
    Purpose
    Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells
    Depositor
    Xiaokun Shu
    Publication
    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FlipGFP(Casp3 cleavage seq) T2A mCherry
    Expression
    Mammalian
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124428-plasmid-map-sequence-id-322471

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  18. pUCM-AAVS1-TO-hNGN2

    Plasmid
    #105840
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron
    Depositor
    Michael Ward
    Publication
    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mCherry
    rtTA3G
    Use
    CRISPR and TALEN
    Promoter
    CAG, EF-1alpha, and TRE3G
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105840-plasmid-map-sequence-id-472335

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  19. pET21b(+)-Is-PETase

    Plasmid
    #112202
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Publication
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112202-plasmid-map-sequence-id-334619

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Showing: 281 - 300 of 66850 results