We narrowed to 8,514 results for: sgrna

1. PX459-sgRNA048_Grb10

   Plasmid

   #232934

   Purpose

   Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Grb10 S. pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKS diaCas9_sgRNA

   Plasmid

   #74923

   Purpose

   Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

   Depositor

   Per Winge

   Article

   Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

   Inserts

   LHCF2 promoter
   diaCas9
   LHCF1 terminator
   U6 promoter
   sgRNA
   U6 3' region
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Cas9 module and sgRNA module

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. U6-sgRNAs-Crym

   Plasmid

   #200067

   Purpose

   For the knock down of Crym with an astrocyte specific mCherry expression

   Depositor

   Baljit Khakh

   Article

   Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

   Insert

   U6-3xsgRNA-Crym
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   GfaABC1D-mCherry

   Expression

   Mutation

   Promoter

   U6, GfaABC1D

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. U6-sgRNA-GFP

   Plasmid

   #200068

   Purpose

   For the knock down of GFP with an astrocyte specific mCherry expression

   Depositor

   Baljit Khakh

   Article

   Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

   Insert

   U6-sgRNA-GFP
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   GfaABC1D-mCherry

   Expression

   Mutation

   Promoter

   U6, GfaABC1D

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HUWE1-sgRNA-1

   Plasmid

   #86924

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA1 (HUWE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

6. HUWE1-sgRNA-2

   Plasmid

   #86925

   Purpose

   To express sgRNA target HUWE1 gene

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   HUWE1 sgRNA2 (HUWE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pH-STEME-1-esgRNA

   Plasmid

   #138135

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEMPTY::sgRNA2

   Plasmid

   #165459

   Purpose

   Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

   Depositor

   Inigo Lasa

   Article

   Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

   Insert

   sgRNA2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   SP01

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH-STEME-NG-esgRNA

   Plasmid

   #138136

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6a:sgRNA(tyr)

    Plasmid

    #64250

    Purpose

    expresses sgRNA(tyr) under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    U6a:sgRNA (tyr)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiGuidPuro-hTP73_sgRNA-1

    Plasmid

    #88855

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pH-PABE-7-esgRNA

    Plasmid

    #115620

    Purpose

    Targeted A to G in rice

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgRNA 2 GAPDH

    Plasmid

    #83809

    Purpose

    Express Sg-2 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPEPZ-sgRNAclone

    Plasmid

    #141090

    Purpose

    This vector is designed for efficient cloning of sgRNAs by Golden Gate assembly. The sgRNA insertion leads to replacement of mCherry, resulting in loss of red color of E. coli colony.

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Cell Host Microbe. 2021 Jan 13;29(1):107

    Insert

    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    p3

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBSV2_Psyn-sgRNA500

    Plasmid

    #149613

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLH-sgRNA1

    Plasmid

    #75388

    Purpose

    sgRNA1

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LENTICRISPR-TKT_sgRNA2

    Plasmid

    #201625

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LENTICRISPR-TKT_sgRNA1

    Plasmid

    #201624

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    TKT (TKT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBbdCas9S_P0526-sgRNAmreB

    Plasmid

    #149654

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid

    #110624

    Purpose

    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    LP1B and hU6

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge