We narrowed to 8,785 results for: sgrna

1. pY2ShHELIX_sgRNA_entry (CJT30)

   Plasmid

   #181781

   Purpose

   Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYLsgRNA-OsU6c

   Plasmid

   #66197

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   OsU6c

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9S_Psyn-sgRNA500

   Plasmid

   #149640

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKDsgRNA-FRT

   Plasmid

   #183241

   Purpose

   anhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda Red

   Depositor

   Karin Schnetz

   Article

   Rangarajan et al Microbiology (Reading). 2022 Apr;168(4).

   Insert

   sgRNA-FRT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P-tet

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9-sgRNA-A+B

   Plasmid

   #149370

   Purpose

   Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites

   Depositor

   Tarek Abbas

   Article

   Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

   Inserts

   Cas9
   sgRNA-A
   sgRNA-B
   Puromycin resistance
   

   Use

   CRISPR

   Tags

   3xFLAG, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh, PGK, and U6

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. psgRNAv1-empty

   Plasmid

   #171611

   Purpose

   sgRNA_v1 plasmid for AsCas12f1 in bacteria

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

   Insert

   AsCas12f1_sgRNA_1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGH020_sgRNA_G418-GFP

   Plasmid

   #85405

   Purpose

   hu6 driven sgRNA vector with G418 and GFP selectable markers

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   G418 resistance and GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYLsgRNA-OsU3m

   Plasmid

   #66193

   Purpose

   cloning of sgRNAs for expression in plants

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   OsU3m (SpeI site destroyed)

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-hEF1A-MG-U6-sgRNA

   Plasmid

   #175505

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a, U6

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MDC1 KO sgRNA

    Plasmid

    #207103

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGTGTAACGTGGAGCCAGTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LENTICRISPR-ALDOA_sgRNA1

    Plasmid

    #201590

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    ALDOA (ALDOA Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LENTICRISPR-UPP1_sgRNA1

    Plasmid

    #201634

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6_rd6_nsgRNA(PP7)

    Plasmid

    #232434

    Purpose

    nsgRNA for PE3b correction of the rd6 mutation, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3b) for rd6 correction driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKDsgRNA-FRT

    Plasmid

    #113398

    Purpose

    expresses gRNA for Cas9 FRT targetting

    Depositor

    Jan Michiels

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    exo, beta, gam, sgRNA-FRT
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. LENTICRISPR-UPP1_sgRNA2

    Plasmid

    #201635

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH-PABE-7-esgRNA

    Plasmid

    #115620

    Purpose

    Targeted A to G in rice

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH-STEME-NG-esgRNA

    Plasmid

    #138136

    Purpose

    Targeted simultaneous C-to-T and A-to-G in rice

    Depositor

    Caixia Gao

    Insert

    APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMPTY::sgRNA2

    Plasmid

    #165459

    Purpose

    Escherichia coli – Staphylococcus aureus shuttle vector for plasmid curing in Gram-positive bacteria

    Depositor

    Inigo Lasa

    Article

    Dorado-Morales et al mBio. 2021 Feb 23;12(1). pii: mBio.03094

    Insert

    sgRNA2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP01

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. KAT5 sgRNA1

    Plasmid

    #138187

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT5

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT5 (KAT5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. KAT5 sgRNA2

    Plasmid

    #138188

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT5

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT5 (KAT5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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