We narrowed to 12,895 results for: otos

1. mCerulean3-EB1-C-18

   Plasmid

   #55415

   Purpose

   Localization: MT End Binding Protein, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pRSM12

   Plasmid

   #101643

   Purpose

   Encodes the fluorescent protein meffCFP for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   meffCFP
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-P1-N-EGFP-C

   Plasmid

   #159436

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-P1-N-miRFP670-C

   Plasmid

   #159438

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Insert

   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSM17

   Plasmid

   #101641

   Purpose

   Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   eqFP486
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EYFP-Clathrin-19

   Plasmid

   #56584

   Purpose

   Localization: Clathrin Vesicles, Excitation: 513, Emission: 527

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. MAC-SARS-CoV-2 NSP7

   Plasmid

   #158358

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   SARS-CoV-2 NSP7 (ORF1ab )
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pJL1-EPA-DNNNS-DQNRT

   Plasmid

   #128397

   Purpose

   In vitro expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Exotoxin A
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   242DNNNS246 and 384DQNRT388 engineered glycosylat…

   Promoter

   T7

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mRFP-dmBACCS2

   Plasmid

   #67630

   Purpose

   Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   mRFP-dmBACCS2
   

   Use

   Drosophila expression

   Tags

   Expression

   Mutation

   Promoter

   Drosophila actin gene 5C gene promoter

   Available since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Nxph4-6A-HA

    Plasmid

    #128103

    Purpose

    Express HA tagged Nxph4-6A

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mVenus-CENPB-N-22

    Plasmid

    #56325

    Purpose

    Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    CENPB (CENPB Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    aa 1-169 of CENPB (DNA Binding Domain)

    Promoter

    CMV

    Available since

    Dec. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. TRIN-E-shREN

    Plasmid

    #105585

    Purpose

    Dox-inducible mirE shREN(control shRNA)/dsRED expression with Venus marker and Neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    control shRNA targeting luciferase
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KAP3-CT

    Plasmid

    #51396

    Purpose

    expression of EGFP-tagged COOH-terminal AA 661–793 KAP3B in mammalian cells

    Depositor

    Trina Schroer

    Article

    Brown et al Traffic. 2005 Dec;6(12):1114-24.

    Insert

    Kinesin-associated protein 3 (KIFAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-Bmp15

    Plasmid

    #163594

    Purpose

    Expresses BMP-15 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp15 (Bmp15 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TRIN-shMYB.2652

    Plasmid

    #105575

    Purpose

    Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    MYB shRNA #2652
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAC-GPR78

    Plasmid

    #158382

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    GPR78 (GPR78 Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. MAC-SARS-CoV-2 NSP8

    Plasmid

    #158359

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP8 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. MAC-SARS-CoV-2 NSP10

    Plasmid

    #158361

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP10 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pJH3666

    Plasmid

    #191447

    Purpose

    Pacr-2s-GCaMP6s::wCherry unc-54 3' UTR C.elegans A/B MN expression of GCaMP6s RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP6s
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2s

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FusionRed-PlastinC-10

    Plasmid

    #56130

    Purpose

    Localization: Cytoskeleton, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Plastin (PLS1 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

21. pGFP-PTEN D92A/C124A

    Plasmid

    #50522

    Depositor

    Kenneth Yamada

    Article

    Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    D92A/C124A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pCAG-Gdf7

    Plasmid

    #163597

    Purpose

    Expresses GDF-7 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf7 (Gdf7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Some Guanin and Cytosine were substituted to redu…

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDestTol2-actinb-kcnj10a-IRES-EGFP

    Plasmid

    #164947

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj10a and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj10a (kcnj10a Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a Gx-NL2

    Plasmid

    #169107

    Purpose

    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMK233 (AAVS1 CMV-AtAFB2)

    Plasmid

    #140537

    Purpose

    AAVS1 CMV-AtAFB2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-AtAFB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-Rab32

    Plasmid

    #31744

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab32 (Rab32 Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Actin

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pERB257

    Plasmid

    #67761

    Purpose

    Expresses KLC1-mCherry-eDHFR

    Depositor

    Michael Lampson

    Article

    Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

    Insert

    KLC1-mCherry-eDHFR (Klc1 Rat)
    

    Use

    Tags

    eDHFR and mCherry

    Expression

    Mammalian

    Mutation

    contains Klc1 amino acids 1-175

    Promoter

    CAG

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAGGs-Hoxc8

    Plasmid

    #45555

    Depositor

    Jeremy Dasen

    Article

    Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

    Insert

    Hoxc8 (HOXC8 Chicken)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pXylA-RgTALsyn

    Plasmid

    #100948

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    XylA (Constitutive)

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge