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AiP11954 - pAAV-hsA2-AiE0078h_3xC2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1954)

Plasmid

#223968

Purpose

AiE0078h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in forebrain glutamatergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-lacZ

Plasmid

#175175

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting lacZ

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

sgRNA-lacZ

Use

AAV and CRISPR

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

Plasmid

#120295

Purpose

AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

C-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

split-intein

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-(n1)QC2-6 FIQ(c4)-P2A-FusionRed

Plasmid

#229573

Purpose

Expresses (n1)QC2-6 FIQ(c4) and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

(n1)QC2-6 FIQ(c4)

Use

AAV

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122

Plasmid

#223184

Purpose

Plasmid containing the Zfr/Firefly luciferase/3xMIR122 cassette. Translation of luciferase is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Inserts

luciferase (LOC116160065 firefly)
mir122 (Mir122 Mouse)

Use

AAV

Tags

HA and engineered P2A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

Plasmid

#203444

Purpose

Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-fDIO-OFF-ArgiNLS-oScarlet

Plasmid

#220606

Purpose

Flp-independent expression of a single-cell discriminating version of oScarlet fluorescent protein. Flp activity can turn off expression.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

oScarlet

Use

AAV

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

Plasmid

#209781

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

cTNT

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

Plasmid

#209782

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

sgRNA

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

Plasmid

#114100

Purpose

fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114104

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

sbGLuc-VChR1-EYFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pKT07_AAV-hU6-AsDR-EFS-Cre-T2A-hNGFR

Plasmid

#256629

Purpose

AAV vector for delivery of AsCas12a guides and expressing Cre and hNGFR using EFS promoter

Depositor

Sidi Chen

Article

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

AAV

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pGW004_pAAV-hU6-SapI-hTBG-Fluc-P2A-Cre

Plasmid

#256631

Purpose

AAV vector for delivery of Cas9 guide and expressing Fluc and Cre using hTBG promoter

Depositor

Sidi Chen

Article

Tang et al Nat Biomed Eng. 2025 Aug;9(8):1290-1308.

Type

Empty backbone

Use

AAV

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ512-pAAV-PB3'IR-CAG-MCP-mtagBFP-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254952

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-mtagBFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-mtagBFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ514-pAAV-PB3'IR-CAG-PCP-mScarlet-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254953

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-mScarlet-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-mScarlet-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ515-pAAV-PB3'IR-CAG-PCP-mtagBFP-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254954

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-mtagBFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-mtagBFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ520-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-hU6-gRNA-PB5'IR

Plasmid

#254955

Purpose

AAV piggyBac vector with single gRNA and CAG-driven MCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ521-pAAV-PB3'IR-CAG-PCP-GFP-KASH-6XPP7-hU6-gRNA-PB5'IR

Plasmid

#254956

Purpose

AAV piggyBac vector with single gRNA and CAG-driven PCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ494-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254949

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-GFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ496-pAAV-PB3'IR-CAG-PCP-GFP-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254950

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ511-pAAV-PB3'IR-CAG-MCP-mScarlet-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254951

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-mScarlet-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-mScarlet-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP11980 - pAAV-DLX2.0-minRho-dTomato-10aa-H2B-WPRE3-BGHpA (Alias: CN1980)

Plasmid

#229934

Purpose

DLX2.0 is an enhancer sequence, designed to drive nuclear dTomato expression in forebrain GABAergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

dTomato-10aa-H2B

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV-RFP-p2A-DAAO-NES

Plasmid

#238920

Purpose

Expression of DAAO with a nuclear export signal and RFP as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003

Plasmid

#234545

Purpose

to express SpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-SC

Use

AAV

Mutation

None

Promoter

mGFAP(ABC1D)

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003

Plasmid

#234546

Purpose

to express dSpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV

Mutation

None

Promoter

mGFAP(ABC1D)

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-mScarlet-optoJNKi-WPRE

Plasmid

#159968

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mScarlet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-Synapsin-Ypet-optoJNKi-WPRE

Plasmid

#159967

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

Ypet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-NOS-IN133.3xFLAG-WPRE

Plasmid

#127869

Purpose

pAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1 (Nos1 Mouse)

Use

AAV

Tags

3xFLAG

Mutation

Amino acids 1-133 only

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVLINKv1-pCALM1-Cre-3'-SCN4A-FLAG

Plasmid

#244365

Purpose

3' AAVLINK plasmid for SCN4A expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

SCN4A (SCN4A Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVLINKv1-pCALM1-Cre-3'-CLTCL1-FLAG

Plasmid

#244337

Purpose

3' AAVLINK plasmid for CLTCL1 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

CLTCL1 (CLTCL1 Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVLINKv1-pCALM1-Cre-3'-GRIN2A-FLAG

Plasmid

#244301

Purpose

3' AAVLINK plasmid for GRIN2A expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

GRIN2A (GRIN2A Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG

Plasmid

#244297

Purpose

3' AAVLINK plasmid for CACNA1C expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

CACNA1C (CACNA1C Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVLINKv1-pCALM1-Cre-3'-TCF20-FLAG

Plasmid

#244285

Purpose

3' AAVLINK plasmid for TCF20 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

TCF20 (TCF20 Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVLINKv1-pCALM1-Cre-3'-SETD1A-FLAG

Plasmid

#244273

Purpose

3' AAVLINK plasmid for SETD1A expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

SETD1A (SETD1A Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVLINKv1-pCALM1-Cre-3'-GRIN2B-FLAG

Plasmid

#244255

Purpose

3' AAVLINK plasmid for GRIN2B expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

GRIN2B (GRIN2B Human)

Use

AAV

Tags

FLAG

Promoter

pCALM1

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DRH031_ AAV-SIO-nEF-WT-THR

Plasmid

#225090

Purpose

Cre-dependent expression of wild-type thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

DN-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
DRH032_ AAV-SIO-nEF-DN-THR

Plasmid

#225091

Purpose

Cre-dependent expression of dominant-negative thyroid receptor beta (THRB, human) in neurons driven by nEF promoter.

Depositor

Bernardo Sabatini

Article

Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

Insert

WT-THRB (THRB Human)

Use

AAV

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP2141 - pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA

Plasmid

#220731

Purpose

AiE2333m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP2017 - pAAV-AiE0774m-minBG-iCre(R297T)-BGHpA

Plasmid

#220723

Purpose

AiE0774m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP2047 - pAAV-AiE2538m-minBG-iCre(R297T)-BGHpA

Plasmid

#220726

Purpose

AiE2538m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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