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We narrowed to 7,695 results for: Pol

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Showing: 2981 - 3000 of 7695 results

  1. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184248-plasmid-map-sequence-id-370322

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  2. pcDNA3-ABKAR(T/A)

    Plasmid
    #140052
    Purpose
    Negative control mutant for ABKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABKAR(T/A)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Nov. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140052-plasmid-map-sequence-id-307468

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  3. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Publication
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Promoter
    TetO Promoter
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

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  4. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79

    Plasmid
    #111725
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q79 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    111725-plasmid-map-sequence-id-226810

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  9. pYeDP60-hATP13A2-WT

    Plasmid
    #171377
    Purpose
    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888
    Depositor
    Peter Vangheluwe
    Publication
    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:
    Insert
    human ATP13A2 variant 2 wild-type (ATP13A2 Human)
    Tags
    BAD tag
    Expression
    Yeast
    Promoter
    URA3
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    171377-plasmid-map-sequence-id-342929

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  11. pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

    Plasmid
    #83461
    Purpose
    Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)
    Depositor
    Thomas Weimbs
    Publication
    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Polycystin-1 (PKD1 Human)
    Tags
    CD16.7
    Expression
    Mammalian
    Mutation
    Amino-acids 4078-4303, R4113A
    Available since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83461-plasmid-map-sequence-id-164733

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  16. pTV001

    Plasmid
    #130265
    Purpose
    EatA expression plasmid containing 8His encoding region in permissive site of eatA gene
    Depositor
    James Fleckenstein
    Publication
    Sheikh et al Infect Immun. 2021 Nov 22:IAI0057221. do
    Insert
    eatA::His8
    Tags
    polyhistidine (internal)
    Expression
    Bacterial
    Mutation
    8His polyhistidine encoding region and XhoI site …
    Promoter
    araBAD
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    130265-plasmid-map-sequence-id-257613

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  17. rat APOBEC1-EGFP

    Plasmid
    #112857
    Purpose
    plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein
    Depositor
    Silvestro Conticello
    Publication
    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112857-plasmid-map-sequence-id-218644

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  18. pGGD_MiniTurboID-YFP

    Plasmid
    #222434
    Purpose
    Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.
    Depositor
    Dominique Bergmann
    Publication
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222434-plasmid-map-sequence-id-440831

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  19. pGGB_YFP-MiniTurboID

    Plasmid
    #222433
    Purpose
    Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.
    Depositor
    Dominique Bergmann
    Publication
    Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MiniTurboID (BirA mutant)
    Use
    Golden gate / green gate compatible cloning vector
    Tags
    Linker and YFP
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    222433-plasmid-map-sequence-id-440826

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  20. CaMKIV-DCTK75E

    Plasmid
    #126423
    Purpose
    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Publication
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed lysine 75 to glutamic acid
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126423-plasmid-map-sequence-id-249663

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Showing: 2981 - 3000 of 7695 results