We narrowed to 11,554 results for: Vars;

1. pRK5 beta1

   Plasmid

   #16042

   Depositor

   Filippo Giancotti

   Article

   Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

   Insert

   beta1 (ITGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCLX-CHOP-dGFP

   Plasmid

   #71299

   Purpose

   Lentiviral plasmid to express destabilized GFP under the control of the ATF-responsive CHOP promoter

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   dGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CHOP (ATF-responsive)

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-P3-evoCjCas9-ABE8e_gRNA

   Plasmid

   #202559

   Purpose

   P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE-RA4.2

   Plasmid

   #208307

   Purpose

   Expresses ABE-RA4.2 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   TadA-RA4.2-SpCas9 D10A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

   Promoter

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSrtA(4S-9) pET29b

   Plasmid

   #75146

   Purpose

   reprogrammed sortase A variant that recognizes alternative substrate LPXSG with high activity and specificity

   Depositor

   David Liu

   Article

   Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   reprogrammed sortase A variant
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   N98D S102C A104V A118T F122A K134R F144L I182V E1…

   Promoter

   T7

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. SGEN

   Plasmid

   #111171

   Purpose

   miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB061i

   Plasmid

   #79226

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-HA4; PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1; PlacIq

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGADT7-αC

   Plasmid

   #197650

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-2 αC
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Ser677T substitution

   Promoter

   ADH1

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GAL-SUV39H2-L

   Plasmid

   #89136

   Purpose

   mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

   Insert

   SUV39H2 (SUV39H2 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2T-CMV-evoCjCas9-BlastR

    Plasmid

    #194059

    Purpose

    Mammalian expression of evoCjCas9 nuclease

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LT3GECIR

    Plasmid

    #111178

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200406

    Purpose

    Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    MCP-ADAR2dd(E488Q)-NES
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    E488Q (hyperactive)

    Promoter

    CMV

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB061i3

    Plasmid

    #79230

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ; PlacIq lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppro1; PlacIq

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDAS12395_PEAR-GFP_2.0-preedited

    Plasmid

    #177185

    Purpose

    PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS_VP35_MARV

    Plasmid

    #135471

    Purpose

    Encodes Marburg virus VP35 gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

    Insert

    VP35
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-Trono-EFS-Blast-P2A-TurboRFP

    Plasmid

    #228893

    Purpose

    Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS_NP_MARV

    Plasmid

    #135470

    Purpose

    Encoding Marburg virus NP gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Schmidt et al J Infect Dis. 2011 Nov;204 Suppl 3:S861-

    Insert

    nucleoprotein (NP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    AG

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBad-mCherry-XL (R125K)

    Plasmid

    #188054

    Purpose

    Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    araBAD

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

    Plasmid

    #153526

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#45

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#45
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LT3REVIR

    Plasmid

    #111176

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge