We narrowed to 9,000 results for: ef1;
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Plasmid#124023PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713DepositorInsertLS-A4_EF1a-NLS::iRFP713-bgPA_R1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB341
Plasmid#124024PurposeEncodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFPDepositorInsertLS-A4_EF1a-NLS::tagBFP-bgPA_R1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK2_025
Plasmid#123717PurposeEncodes crippled human pEF1alpha as a Type 2 part to be used in the MTK systemDepositorInsertpEF1ac
UseTagsExpressionMammalianMutationCrippled Kozac sequencePromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF736
Plasmid#121893PurposeEF1a-dTEV-protease-GFPDepositorInsertsTEV protease
eGFP
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p415TEF-NQYFP
Plasmid#99554PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorInsertSwi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterTEF1Available sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
attB-puro donor.dna
Plasmid#181923PurposeattB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertionDepositorInsertattB-EGFP-EF1α-PuroR-T2A-TagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet3-CAAX-IRES-Puro
Plasmid#222639PurposeLive-cell plasma membrane markerDepositorInsertmScarlet3-CAAX
UseLentiviralTagsmScarlet3ExpressionMutationPromoterEF1aAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10704
Plasmid#183957PurposeU6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFPDepositorInsertSKSKSO array
UseLentiviralTagsEF1a-Puro-T2A-TagBFPExpressionMammalianMutationPromoterU6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-cyan pre-eGRASP(p32)
Plasmid#111589PurposeAn AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.DepositorInsertcyan pre-eGRASP(p32)
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II-dCas13-APO1-YTH
Plasmid#204767PurposeGEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)DepositorInsertdCas13-NLS-APO1-YTH-HA
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEf1alphaAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ampl43
Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterTEF1Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER-HA-mNG21-10
Plasmid#141165PurposeSplit Fluorescent Reporter for ER Retrograde TraffickingDepositorInsertER-HA-mNG21-10-KDEL
UseLentiviralTagsSignal Sequence, KDEL and HAExpressionMammalianMutationK128M, S142T, R150M, G172V and K213MPromoterEf1aAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HIVGKO
Plasmid#112234PurposeHIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1aDepositorInsertcsGFP-EF1a-mKO2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfralpha-EGFPN
Plasmid#66787PurposeExpression of murine PDGFRalpha tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRalpha (Pdgfra Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
phMYT1L-N106
Plasmid#60861PurposeExpresses MYT1L under the EF1a promoterDepositorInsertMYT1L (MYT1L Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EWB-DIO-cyan pre-eGRASP(p30)
Plasmid#111583PurposeAn AAV vector that expresses double floxed cyan pre-eGRASP(p30) under the Ef1a promoter.DepositorInsertcyan pre-eGRASP(p30)
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
SZ_EC_02
Plasmid#155341PurposeOverexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XRDepositorInsertstef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator
UseEasycloneyali insert vectorTagsExpressionYeastMutationPromotergapdh, pyk1, and tef1Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Puro-24xMS2
Plasmid#212629PurposeMS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particlesDepositorInsertEF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-HA-mNG21-10
Plasmid#141213PurposeMembrane Tethered Split Fluorescent Reporter for ER RetragradeDepositorInsertSec61b-HA-mNG21-10
UseLentiviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2793
Plasmid#64043PurposepCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxTagsExpressionYeastMutationPromoterTEF1 promoter and noneAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only