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pCMV_NFL(WT)-FLAG

Plasmid

#182654

Purpose

Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Publication

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

wild type mouse neurofilament light chain (Nefl Mouse)

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-S650D

Plasmid

#171246

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)

Depositor

Steven Balk

Publication

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Ser650Asp (AR-S650D)

Promoter

CMV

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-S650A

Plasmid

#171245

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)

Depositor

Steven Balk

Publication

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Ser650Ala (AR-S650A)

Promoter

CMV

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+(Zeo)-FLAG-muGFP

Plasmid

#166726

Purpose

Expresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cells

Depositor

Daniel Scott

Publication

Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

muGFP

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

Plasmid

#52502

Purpose

expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO11 (FBXO11 Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

deletion of Fbox (deleted aa 71-116)

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Flag‐mTim1 (Bl/6)

Plasmid

#49205

Purpose

expresses Flag tagged mouse Tim1

Depositor

Lawrence Kane

Publication

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (BL/6 allele) (Havcr1 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLJM60 FLAG-RAB35 S22N

Plasmid

#107106

Purpose

Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

Depositor

Charles Sawyers

Publication

Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

Insert

RAB35 (RAB35 Human)

Use

Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin P1

Plasmid

#68816

Purpose

Flag tagged Geph mammalian expression

Depositor

Shiva Tyagarajan

Publication

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyrin…

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA flag-Msi1

Plasmid

#184029

Purpose

Msi1 clone with N-terminal flag tag

Depositor

Peter Stoilov

Publication

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Msi1 (Msi1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-H3.3K4M-FLAG/HA

Plasmid

#128741

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Publication

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Mutation

K4M

Promoter

MSCV

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag-nls-NgAgo-GK

Plasmid

#86435

Purpose

Expresses a Flag tag on the N-terminal

Depositor

Gaétan Burgio

Publication

Khin et al PLoS One. 2017 Jun 13;12(6):e0178768. do

Insert

NgAgo

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-3xFLAG-His6-LARP1

Plasmid

#233626

Purpose

Expression of Flag- and His-tagged LARP1

Depositor

Shintaro Iwasaki

Publication

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

LARP1 (LARP1 Human)

Tags

3FLAG-His6

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FNTA-FLAG

Plasmid

#86858

Purpose

To express FNTA with a FLAG tag at C terminus.

Depositor

Yihong Ye

Publication

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Tags

FLAG

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xFlag

Plasmid

#67170

Purpose

CRISPaint universal donor for flag fusion tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

3xFlag

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3C-3xFlag

Plasmid

#99264

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

Depositor

Alejandro Adam

Publication

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Mutation

A662C N664C

Promoter

CMV

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLJM1-Flag-Rheb

Plasmid

#165031

Purpose

expression of Flag tagged Rheb protein in mammalian cells

Depositor

Ken Inoki

Publication

Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

Insert

Rheb (RHEB Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCI-FLAG-mTUT4(D1028A)

Plasmid

#60220

Purpose

Mammalian expression of FLAG-tagged catalytic mutant of TUT4

Depositor

Zissimos Mourelatos

Publication

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT4 (Tut4 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

D1028A

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-C9orf114-flag

Plasmid

#70647

Purpose

Expresses C9orf114 with FLAG-tag epitope.

Depositor

David Sabatini

Publication

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

C9orf114 (SPOUT1 Human)

Use

Retroviral

Tags

3X FLAG

Expression

Mammalian

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

Plasmid

#58492

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)

Depositor

David Ron

Publication

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

deleted amino acids P29 to D408 and bearing mutat…

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

Plasmid

#149714

Purpose

Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

Depositor

Silvia Monticelli

Publication

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Promoter

EF1α

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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