We narrowed to 5,481 results for: PID

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pJ3 SHP2 WT

Plasmid

#8317

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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MTK0_062

Plasmid

#123951

Purpose

Encodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR DEST

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mKate2-B_UTR

Plasmid

#68480

Purpose

Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKate2-B_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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mEmerald-PDHA1-N-10

Plasmid

#54225

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

PDHA1 (PDHA1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGDP4 ermC

Plasmid

#112884

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

Depositor

Gerard Wright

Article

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

ermC (ermM Staphylococcus epidermis)

Tags

6xHis

Expression

Bacterial

Promoter

Plac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRDB_156

Plasmid

#216090

Purpose

Cas9 [Sp] CRISPRa targeting CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD274 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR tdTomato L5-L2

Plasmid

#62185

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

tdTomato

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR MCS L1-L4

Plasmid

#62087

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL703

Plasmid

#140373

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-2BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

Plasmid

#190794

Purpose

Template vector to amplify pieces for creating multiplex sgRNA-arrays.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA-scaffold only

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2319

Plasmid

#195697

Purpose

Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

Foldon-MlaC

Expression

Bacterial

Mutation

6xHis-Foldon-(Gly-Ser-Linker)-MlaC

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pIRES-Ap2a2-FKBP

Plasmid

#46944

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap2a2-FKBP

Tags

Mainly mouse Ap2a2 cDNA, with a fragment of brain…

Expression

Mammalian

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Plasmid

#219781

Purpose

CaST-P2A for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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NFAST(low)-mApple-mito

Plasmid

#214415

Purpose

Mitochondria localization of low-affinity NFAST fused to mApple-mito

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Tags

mApple-mito

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pME-p3E-HyPer7

Plasmid

#194289

Purpose

Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

Hyper7

Use

Multisite gateway entry clone

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

Plasmid

#199363

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Sec61b-HA-mNG21-10

Plasmid

#141213

Purpose

Membrane Tethered Split Fluorescent Reporter for ER Retragrade

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

Sec61b-HA-mNG21-10

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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CFP-GAI(1-172)

Plasmid

#37309

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

CFP

Expression

Mammalian

Mutation

contains aa 1-172

Promoter

CMV

Available Since

June 19, 2012

Availability

Academic Institutions and Nonprofits only
Venus-1-155-Ggamma2

Plasmid

#234752

Purpose

Mammalian expression of BiFC Venus-betagamma (with Venus-155-239-Gbeta1)

Depositor

Nevin Lambert

Article

Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

Insert

GNG2 (GNG2 Human)

Tags

VC155

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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