We narrowed to 40,146 results for: kan

1. pRT498-OtUBD

   Plasmid

   #190089

   Purpose

   Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK286 (mAID-NeoR)

   Plasmid

   #72824

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Neo
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pECIA2

   Plasmid

   #47032

   Purpose

   This is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.

   Depositor

   Chris Garcia

   Article

   Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

   Type

   Empty backbone

   Use

   Secreted expression in drosophila culture

   Tags

   Fc(human IgG1), HRV 3C Protease Cleavage site, He…

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   Aug. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMALp2x-CARP-1

   Plasmid

   #41727

   Depositor

   Koji Okamoto

   Article

   Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

   Insert

   clam ADP-ribosylating protein-1
   

   Use

   Bacterial subcloning

   Mutation

   deleted 5'-UTR nucleotides 1-64

   Promoter

   pMB1

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pSCM001

   Plasmid

   #124605

   Purpose

   Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

   Depositor

   David Summers

   Article

   Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

   Insert

   mCherry-pHluorin
   

   Tags

   mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

   Expression

   Bacterial

   Promoter

   pDAB

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDOC-GG

   Plasmid

   #149377

   Purpose

   Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

   Depositor

   Mark Pallen

   Article

   Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

   Inserts

   Fragment containing a kanamycin resistance cassette
   Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
   Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
   Fragment containing an I-SceI recognition sequence
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSecTag-ATX

   Plasmid

   #17839

   Depositor

   Steven Rosen

   Article

   Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

   Insert

   Autotaxin (ENPP2 Human)
   

   Expression

   Mammalian

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-Syn-VARNAM2

   Plasmid

   #195520

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   Synapsin

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJY-RpABE

   Plasmid

   #112872

   Purpose

   binary vector for ABE expression in Arabidopsis

   Depositor

   Jin-Soo Kim, Jae-Young Yun

   Article

   Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

   Inserts

   RPS5A promoter
   ABE7.10
   U6-sgRNA cassette
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXYB089

    Plasmid

    #239628

    Purpose

    Plant RNA Vision constructs with no gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01
    stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXYB082

    Plasmid

    #239578

    Purpose

    Plant RNA Vision constructs with 41 bp gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA01
    gRNA02-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXYB083

    Plasmid

    #239579

    Purpose

    Plant RNA Vision constructs with 82 bp gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA03
    gRNA04-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK288 (mAID-Bsr)

    Plasmid

    #72826

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Bsr
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

    Plasmid

    #129537

    Purpose

    PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    tetR-3xFlag-HA-2A-Puro
    

    Use

    Synthetic Biology

    Promoter

    hPGK promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. hGATA-1 WT

    Plasmid

    #118352

    Purpose

    This plasmid expresses human GATA-1 WT.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQAT27

    Plasmid

    #223399

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. p52K-GFP-KS

    Plasmid

    #237690

    Purpose

    For overexpression of 52K-GFP-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    52K-GFP-KS
    

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTYF-super-mfPENK-0.9k-Venus-WPRE

    Plasmid

    #156401

    Purpose

    Expresses Venus under PENK gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMK281 (mCherry2-Hygro)

    Plasmid

    #72797

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Hygro
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTYF-super-mfSST-0.3k-Venus-WPRE

    Plasmid

    #156393

    Purpose

    Expresses Venus under SST gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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