We narrowed to 13,061 results for: otos

1. pAAV-CaMKII-Mac-GFP

   Plasmid

   #58849

   Purpose

   AAV expression of Mac-GFP under the CaMKII promoter

   Depositor

   Edward Boyden

   Article

   Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

   Insert

   Mac-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT7-AsCas12a_crRNA-site3 (RTW549)

   Plasmid

   #160138

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL421_MLL2_promoter

   Plasmid

   #118696

   Purpose

   Express MLL2/KMT2B promoter for luciferase assay

   Depositor

   Christopher Vakoc

   Article

   Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

   Insert

   MLL_promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TB:2xP53_RE:MiniP

   Plasmid

   #133881

   Purpose

   2 copies of the p53 DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   2 copies of the p53 DNA binding motif
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pG.Lantern-SM50-GFP

   Plasmid

   #135593

   Purpose

   Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

   Depositor

   Mamiko Yajima, Koji Akasaka

   Article

   Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

   Insert

   SM50 promoter (SM50 S. purpuratus)
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   Green Lantern GFP

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRII-Topo Foxp2 in situ probe

   Plasmid

   #45620

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

   Insert

   Foxp2 in situ probe (Foxp2 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains bp#1709-2142 of AF339106

   Promoter

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pNMHCII-C1C2-GFP

   Plasmid

   #46044

   Depositor

   Robert Adelstein

   Article

   Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

   Insert

   nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available since

   Aug. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

   Plasmid

   #163610

   Purpose

   Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   cofilin1 (Cfl1 Mouse)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSET FLIPglu-600uDelta11 Ares-Aphrodite

   Plasmid

   #13571

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A Delta11 Aphrodite
   

   Use

   Tags

   Aphrodite and Ares

   Expression

   Bacterial

   Mutation

   Fluorophore-derived linker shortened. F16A mutati…

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

    Plasmid

    #170811

    Purpose

    Lentiviral vector to co-express an sasgRNA with NLS-mNeonGreen

    Depositor

    Kenneth Yamada

    Article

    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCerulean3-PCNA-19-SV40NLS-4

    Plasmid

    #55437

    Purpose

    Localization: Nucleus, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)

    Plasmid

    #40341

    Depositor

    Alexander Dent

    Article

    Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

    Insert

    Blimp1 promoter (mutated) (PRDM1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Two AP-1 sites at -1813 and -1647 replaced with B…

    Promoter

    Blimp1

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

13. (369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)

    Plasmid

    #168190

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nLag6-(EAAAK)4-OGA(544-706)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBW105

    Plasmid

    #111137

    Purpose

    For the expression of rat AANAT with Met-Leu-Ser N-terminus in S. cerevisiae

    Depositor

    Alexander Varshavsky

    Article

    Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

    Insert

    MLS-ratAANAT-3FLAG (Aanat Rat)
    

    Use

    Tags

    3x Flag

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET22b-ecDHFR-Y100F

    Plasmid

    #110826

    Purpose

    Bacterial expression of E.coli DHFR Y100F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

    Insert

    ecDHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y100 mutated to F100

    Promoter

    T7

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSET FLIPglu-600uDelta13 Aphrodite

    Plasmid

    #13570

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta13 Aphrodite
    

    Use

    Tags

    Aphrodite and ECFP

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRII-Topo Cux2 in situ probe

    Plasmid

    #45623

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Cux2 in situ probe (Cux2 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains nt 1069-1694 on NM_007804

    Promoter

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)

    Plasmid

    #70194

    Purpose

    Derived from 21 with silent mutations to make BsaI, BbsI compatible

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-tetpA-iRPT

    Plasmid

    #140763

    Purpose

    Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with a TRE cassette

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

    Inserts

    iRFP713
    PuroR
    rtTAM2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. K347CLM G234A/43/347

    Plasmid

    #24454

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, G234A/S43C/E3…

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

21. TRIN-E-shTAF12.376

    Plasmid

    #105573

    Purpose

    Dox-inducible mirE TAF12 shRNA/dsRED expression with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 shRNA
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

22. pLVTHM-shREV3-4

    Plasmid

    #38033

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

    Insert

    shREV3-4 (Rev3l, Rev3l, REV3L Rat, Mouse, Human, human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pBS chick PEA3 [TJ#51]

    Plasmid

    #16279

    Depositor

    Thomas Jessell

    Article

    Lin et al Cell. 1998 Oct 30. 95(3):393-407.

    Insert

    PEA3 (ETV4 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pBS chick ER-81 [TJ#50]

    Plasmid

    #16274

    Depositor

    Thomas Jessell

    Article

    Lin et al Cell. 1998 Oct 30. 95(3):393-407.

    Insert

    ER81 (ETV1 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

25. mCerulean3-H2B-C-10

    Plasmid

    #55422

    Purpose

    Localization: Nucleus/Histones, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

26. TRIN-shMYB.2572

    Plasmid

    #105574

    Purpose

    Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    MYB shRNA #2572
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PCDNA3-N3FLAG-MYB-(1-327)

    Plasmid

    #105612

    Purpose

    transient express MYB N terminal fragment with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    N terminal fragment of MYB (mouse), amino acids 1-327 (Myb Mouse)
    

    Use

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PCDNA3-N3FLAG-MYB-DBD(1-200)

    Plasmid

    #105610

    Purpose

    transient express MYB DBD fragment with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    DBD fragment of MYB (mouse), amino acids 1-200 (Myb Mouse)
    

    Use

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pES005 (pTet-qacR wt/pQacA-deGFP)

    Plasmid

    #69034

    Purpose

    reporter with wild-type qacR on single plasmid

    Depositor

    Richard Murray

    Article

    de Los Santos et al ACS Synth Biol. 2015 Aug 19.

    Inserts

    qacR (qacR Synthetic)
    deGFP
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    pQacA and pTet

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOGG113

    Plasmid

    #133124

    Purpose

    pL0M-PU-NoP, promotorless region used as negative control

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    NoP promotorless region from plasmid pIJJ11268
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge