We narrowed to 11,224 results for: NSI

1. pcDNA3-Lyso-ExRai-AktAR2

   Plasmid

   #184052

   Purpose

   Lysosome-targeted ExRai-AktAR2.

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   Lyso-ExRai-AktAR2
   

   Use

   Tags

   Lysosome-associated membrane protein 1 (LAMP1).

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138128

   Purpose

   Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mClover3-anti-mouse-IgG(kappa)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 3xFLAG-dCas9/pMXs-neo

   Plasmid

   #51260

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191156

   Purpose

   Expression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:GAPDH.s1:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SuExp-hPLD4

   Plasmid

   #201244

   Purpose

   express full length human PLD4 protein

   Depositor

   David Nemazee

   Article

   Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

   Insert

   PLD4 (PLD4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR-p38KTRClover

   Plasmid

   #59142

   Purpose

   ORF encoding p38 KTR mClover flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBp-FGFR3c-WT

   Plasmid

   #45711

   Depositor

   Matthew Meyerson

   Article

   Liao et al Cancer Res. 2013 Jun 20.

   Insert

   FGFR3 IIIc (FGFR3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV7.1_3xFLAG-TAOK3

   Plasmid

   #172993

   Purpose

   constitutive expression of FLAG-tagged TAOK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-TAOK3 (TAOK3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-N1-FIH1

   Plasmid

   #21403

   Depositor

   Eric Metzen

   Article

   Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

   Insert

   HIF1AN (HIF1AN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pQCXIH-myc-hTAZ

    Plasmid

    #32841

    Depositor

    Kunliang Guan

    Article

    Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/MSV

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pMJA103

    Plasmid

    #128519

    Purpose

    Expresses human amyloid Lysozyme in Pichia pastoris

    Depositor

    Marcos Alcocer

    Article

    Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

    Insert

    Artificial gene for human lysozyme D67H (LYZ Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. tdp43-EGFP construct2

    Plasmid

    #28195

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa86-414 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pCAGGS-CRY2-mCherry-MYPT169

    Plasmid

    #178526

    Purpose

    Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CRY2-mCherry-MYPT169
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAS_FLAG-G1 PylRS

    Plasmid

    #154763

    Purpose

    plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200403

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM

    Plasmid

    #183150

    Purpose

    Expression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Inserts

    Lex-ER-B112
    Zif268 DBD - hPRLBD - MSN2AD
    

    Use

    For yeast genomic integration

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEX-CFP-hLC3WT

    Plasmid

    #24985

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a wild type human MAP1-LC3B fused with ECFP (MAP1LC3B Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-NPC1(P202A/F203A)-FLAG

    Plasmid

    #164976

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    P202A/ F203A

    Promoter

    EF1a

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

    Plasmid

    #174892

    Purpose

    amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-PB2-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187916

    Purpose

    Expresses C terminal 3xFLAG tagged PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 2
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pWP1_mHAL

    Plasmid

    #102318

    Purpose

    expression of mouse cDNA of HAL refractory to the guide in plasmid 4

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Hal (Hal Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLVX-mCherry-SPASTIN

    Plasmid

    #180647

    Purpose

    Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, has siRNA resistance to GAACAGUGUG…

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-FLAG-Sestrin1 isoform1

    Plasmid

    #72594

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN1 (SESN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

24. mEos3.2GPI

    Plasmid

    #203759

    Purpose

    Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    CD58 c-term (CD58 Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AA299

    Plasmid

    #216029

    Purpose

    Fragmid fragment: (Cas protein) NNGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Slug]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tdp43-EGFP construct5

    Plasmid

    #28198

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa257-414 only

    Promoter

    Available since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

27. LentiCRISPRv2 EGFP sgRNA1

    Plasmid

    #164932

    Purpose

    Expresses EGFP sgRNA1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBabe WT Akt2

    Plasmid

    #14551

    Depositor

    Joan Brugge

    Article

    Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

    Insert

    Akt2 (AKT2 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

29. B1-INTEGRIN VN

    Plasmid

    #195216

    Purpose

    Mammalian expression of mCherry tagged B1 Integrin. Autoclustering mutant.

    Depositor

    Valerie Weaver, David Bryant

    Article

    Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V737N

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEX-GFP-hLC3ΔG

    Plasmid

    #24988

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a mutant human MAP1-LC3B fused with EGFP (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted Gly at 121

    Promoter

    Available since

    June 28, 2010

    Availability

    Academic Institutions and Nonprofits only

31. VP16-hAR

    Plasmid

    #89092

    Purpose

    mammalian expression of full-length human androgen receptor with added N-terminal VP16 activation domain

    Depositor

    Elizabeth Wilson

    Article

    Langley et al J Biol Chem. 1998 Jan 2;273(1):92-101.

    Insert

    hAR (AR Human)
    

    Use

    Tags

    VP16

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

32. ABCA1 promoter luciferase reporter (Full-length)

    Plasmid

    #86442

    Purpose

    Human ABCA1 promoter luciferase reporter (Full-length)

    Depositor

    David Mu

    Article

    Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

    Insert

    ABCA1 promoter (ABCA1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA-FIH1

    Plasmid

    #21399

    Depositor

    Eric Metzen

    Article

    Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

    Insert

    HIF1AN (HIF1AN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

35. pHJ4: LexA-VHH-Caffeine

    Plasmid

    #108242

    Purpose

    LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    LexA DNA binding domain fusing with VHH-Caffeine antibody
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO1

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV-TetOn-Cdt1-mCherry

    Plasmid

    #193760

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    WT

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. lentiCRISPR v2-sgMyc_1

    Plasmid

    #138324

    Purpose

    Express guide RNA 2 for mouse Myc

    Depositor

    Yi Zhang

    Article

    Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

    Insert

    cMyc sgRNA (Myc Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187918

    Purpose

    Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 1
    

    Use

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEX-HcRed-hLC3WT

    Plasmid

    #24991

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a wild type human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pRK5-FLAG-Sestrin3

    Plasmid

    #72592

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN3 (SESN3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only