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pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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NPC1-CFP P692S

Plasmid

#58457

Purpose

expression of NPC1-CFP sterol sensing domain mutant

Depositor

Matthew Scott

Article

Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

Insert

NPC1 (Npc1 Mouse)

Tags

ECFP and His6

Expression

Mammalian

Mutation

P692S sterol sensing domain mutant

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-Kdm4b-pA

Plasmid

#104486

Purpose

Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

Depositor

Yi Zhang

Article

Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

Insert

Kdm4b (Kdm4b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD1

Plasmid

#153116

Purpose

Expression of Flag tagged human FOXD1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD1 (FOXD1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-Tollip

Plasmid

#178050

Purpose

GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP

Plasmid

#119903

Purpose

Bxb1 integration plasmid for cuO array with Puro selection cassette

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

cuOx144 array

Use

Mouse Targeting

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-UBF

Plasmid

#26672

Depositor

Sui Huang

Article

Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

Insert

UBF1 (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pmPB3-VSV

Plasmid

#68039

Purpose

Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B3 (Plxnb3 Mouse)

Tags

VSV

Expression

Mammalian

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pGL2-Basic-Myogenin core promoter

Plasmid

#134722

Purpose

Myogenin core promoter in a luciferase reporter plasmid

Depositor

Michael Chin

Article

Sun et al J Biol Chem. 2001 May 25;276(21):18591-6

Insert

Myogenin Promoter

Use

Luciferase

Expression

Mammalian

Promoter

Myogenin

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-Skp2

Plasmid

#19947

Depositor

Yue Xiong

Article

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

S-phase kinase-associated protein 2 (p45) (SKP2 Human)

Tags

myc

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-TREX1(D18N)

Plasmid

#27220

Depositor

Judy Lieberman

Article

Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

Insert

TREX1 (TREX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

D18N sequence 52-GAC changed to AAC, resulting i…

Available Since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

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FEOX-PBCS-TBF2- MC2

Plasmid

#234406

Purpose

Tool for genetically encoded fluorescent biosensor of cellular iron microenvironment

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Sci Rep. 2025 Oct 21;15(1):36596. doi: 1

Inserts

FEOX1 CONTROL
FEOX

Use

Synthetic Biology

Tags

mTagBFP2

Expression

Mammalian

Promoter

EF1-alpha and hpgk

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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G555

Plasmid

#216483

Purpose

Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

Depositor

Christophe Danelon

Article

van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021

Insert

plsB, plsC, cdsA, pssA

Use

Cell-free expression

Promoter

T7 for each gene

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMK1221

Plasmid

#84220

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherry

Depositor

Martin Kampmann, Jonathan Weissman

Article

Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSL4393 (pClpX_Eco)

Plasmid

#200907

Purpose

Expresses human codon-optimized E. coli ClpX from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

EcoClpX

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pROSA26-CreERT2

Plasmid

#149437

Purpose

ROSA26 targetting vector with tamoxifen-inducible Cre recombinase

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

ERT2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSL3713 (pTnsC_Pse)

Plasmid

#200900

Purpose

Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseTnsC

Tags

3xNLS

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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hNCAM2

Plasmid

#84473

Purpose

expression of hNCAM2 in mammalian cells

Depositor

Vladimir Sytnyk

Article

Leshchyns'ka et al Nat Commun. 2015 Nov 27;6:8836. doi: 10.

Insert

NCAM2 (NCAM2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-GFP-HiBiT

Plasmid

#198317

Purpose

Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-GFP-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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