We narrowed to 10,873 results for: Kars;

1. pMIIS928

   Plasmid

   #238211

   Purpose

   Expresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Use

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMIIS793

   Plasmid

   #238206

   Purpose

   Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

   Depositor

   Feng Zhang

   Article

   Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

   Insert

   TaTasR
   

   Use

   Tags

   TwinStrepII-bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPRa_all-in-one

   Plasmid

   #183695

   Purpose

   Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-Flag-hsDicer

   Plasmid

   #41584

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA GFP10-ZIP

   Plasmid

   #182241

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP10

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   GFP10-ZIP
   

   Use

   Tags

   split-GFP10 tripartite

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA ZIP-GFP11

   Plasmid

   #182242

   Purpose

   Tripartite split-GFP mammalian. Express leucine zipper with GFP11

   Depositor

   Stéphanie Cabantous

   Article

   Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

   Insert

   ZIP-GFP11
   

   Use

   Tags

   split-GFP11 tripartite

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUGW-ER-TurboID

   Plasmid

   #200965

   Purpose

   3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   ER-TurboID
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. amilCP chromoprotein

   Plasmid

   #117847

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilCP
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PB_iMYOD1_P2A_GFP_Puro

    Plasmid

    #168804

    Purpose

    PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    MYOD1 (MYOD1 Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA GFP10-ZIP-GFP11

    Plasmid

    #182243

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP-11
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. amilCP chromoprotein promoter-less

    Plasmid

    #118008

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-mito-mTagBFP2

    Plasmid

    #216130

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pY010 (pcDNA3.1-hAsCpf1)

    Plasmid

    #69982

    Purpose

    Expresses humanized AsCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hAsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET51b-His-TEV-HaloTag7

    Plasmid

    #167266

    Purpose

    Overexpression of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP VAMP3

    Plasmid

    #42310

    Depositor

    Thierry Galli

    Article

    Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

    Insert

    VAMP 3 (VAMP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDF0159 pCMV - huDisCas7-11 mammalian expression

    Plasmid

    #172507

    Purpose

    Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pCMV - huDisCas7-11 mammalian expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a - Ec-ClpX

    Plasmid

    #111516

    Purpose

    Expresses E. coli ClpX with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    ClpX
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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