We narrowed to 12,189 results for: LEA
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Plasmid#119758PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.DepositorTypeEmpty backboneTagsGST and Strep-tag II fusion tagExpressionBacterialPromotertac promoterAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only
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pCAGG-NFIX2
Plasmid#112699PurposeExpression of NFIX2 protein and GFPDepositorAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)
Plasmid#30184DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…ExpressionBacterialAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmCer-Mcm5
Plasmid#211391Purposemammalian expression of human Mcm5 N-terminally fused to mCeruleanDepositorAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CalfluxCTN
Plasmid#190068PurposeExpresses BRET Ca2+ sensor CalfluxCTN in mammalian cellsDepositorInsertCalfluxCTN
ExpressionMammalianPromoterCAG promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIVT-dCas9-mCherry
Plasmid#174443Purposein vitro transcription template for dCas9-mCherryDepositorInsertCas9 Endonuclease Dead
UseIn vitro transcription templateTagsmCherryPromoterT7 SP6Available sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p2T-CMV-AIDmax-BlastR
Plasmid#152994PurposeC-to-T base editorDepositorInsertAID-nSpCas9-UGI-UGI
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE80L TriCatcher-ELP
Plasmid#112630PurposeTwo SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcherDepositorInsertTriCatcher-ELP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
NKP38 GFP (1009)
Plasmid#62021Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pL0M-S-mNeonGreen-EC18153
Plasmid#137075PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY019 (pcDNA3.1-hPmCpf1)
Plasmid#69991PurposeExpresses humanized PmCpf1DepositorInserthPmCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY015 (pcDNA3.1-hLiCpf1)
Plasmid#69987PurposeExpresses humanized LiCpf1DepositorInserthLiCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY009 (pcDNA3.1-hSsCpf1)
Plasmid#69981PurposeExpresses humanized SsCpf1DepositorInserthSsCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXcX-PRS-EGFP
Plasmid#25865DepositorInsertPRS driven enhanced green fluorescent protein
ExpressionMammalianAvailable sinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN6kwh
Plasmid#44724DepositorInsertspCMV-D2i promoter
htetR::NLS
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOCC240
Plasmid#118895Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and mCherry, clea…ExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
CuO-LacI-NLS–SNAPf
Plasmid#227368PurposeLentiviral expression of LacI–SNAPDepositorInsertLacI NLS SNAPf
UseLentiviralExpressionMammalianAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2
Plasmid#167295PurposePlasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherryDepositorInsertBAK (BAK1 Human)
ExpressionMammalianAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVH-EF1a-GFP-P2A-KLF4
Plasmid#130694PurposeLentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.DepositorInserthuman KLF4 (KLF4 Human)
UseLentiviralAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by