We narrowed to 18,569 results for: MUT

1. pET28a-PTBP1-RRM12CCtoSS

   Plasmid

   #89155

   Purpose

   Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
   

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-CMV-cGAS R71/75E C396/7A-HA

   Plasmid

   #130920

   Purpose

   Expresses human cGAS with R71/75E and C396/397A point mutations; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   human cGAS R71/75E C396/7A
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa 71 and 75 mutated to glutamate; aa 396 and 397…

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-FLEXfrt-SaCas9-U6-sgGria3

   Plasmid

   #124869

   Purpose

   Mutagenesis of Gria3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria3 (Gria3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.2-Cx43-ML-HA

   Plasmid

   #49857

   Purpose

   Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   All internal Methionines mutated to Leucine. Cont…

   Promoter

   CMV

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCEP4-CCR5(R5gp120-Hi)

   Plasmid

   #98954

   Purpose

   Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Tags

   Signal/leader sequence from Influenza A virus HA …

   Expression

   Mammalian

   Mutation

   R5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q…

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5 TO FRT Myc ArB K106R

   Plasmid

   #59807

   Purpose

   Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora B (AURKB Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K106R, kinase mutant

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HNF4A_P2-285/-192

   Plasmid

   #31053

   Depositor

   Gerhart Ryffel

   Article

   Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   -285 to -1 of P2 HNF4A promoter with mutation -19…

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HNF4A_P2-285/-169

   Plasmid

   #31052

   Depositor

   Gerhart Ryffel

   Article

   Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   -285 to -1 of P2 HNF4A promoter with mutation -16…

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HNF4A_P2-285/-136

   Plasmid

   #31051

   Depositor

   Gerhart Ryffel

   Article

   Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

   Insert

   HNF4A P2 promoter (HNF4A Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   -285 to -1 of P2 HNF4A promoter with mutation -13…

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pD10AH840AhCas9 (GB1041)

    Plasmid

    #68207

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Tau BA 12->10 N296N

    Plasmid

    #203383

    Purpose

    For transfection and in vitro assay

    Depositor

    Stefan Stamm

    Article

    Margvelani et al Biochim Biophys Acta Mol Basis Dis. 2024

    Insert

    MAPT (MAPT Human)
    

    Expression

    Mammalian

    Mutation

    N296N (silent mutation)

    Promoter

    CMVf

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIDTSmart-CMV-PLRS1

    Plasmid

    #217365

    Purpose

    Expression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"

    Depositor

    Abhishek Chatterjee

    Article

    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2

    Insert

    leucyl-tRNA synthetase polyspecific mutant
    

    Expression

    Mammalian

    Mutation

    M40I, T252A, Y499I, Y527A, H529G

    Promoter

    CMV

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pdnCas3-CDA1

    Plasmid

    #229535

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    dnCas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pdnCas3-APOBEC1

    Plasmid

    #229537

    Purpose

    pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    dnCas3
    Uracil glycosylase inhibitor
    

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid

    #191572

    Purpose

    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    SARS-CoV-2 Spike Protein (S Synthetic)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    D614G and F486S (confers reduced binding affinity…

    Promoter

    CMV

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGM84

    Plasmid

    #220172

    Purpose

    CRISRPi guide RNA against BTF3

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    BTF3 (sgRNA) (BTF3 Human)
    

    Use

    Lentiviral

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cav2.1 R57A, R59A pMT2

    Plasmid

    #206112

    Purpose

    expression of rat Cav2.1 calcium channel with R57A and R59A mutations that prevent G-protein modulation

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ACVR1-TASA-R206H

    Plasmid

    #206182

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S190A, S194A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ACVR1-TSAA-R206H

    Plasmid

    #206183

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S192A, S194A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S192A, S194A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ACVR1-VSAS-R206H

    Plasmid

    #206185

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations T189V, S192A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    T189V, S192A, R206H

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge