We narrowed to 29,768 results for: REP

1. pMK446 (BSD-P2A-BromoTag-mClover)

   Plasmid

   #214376

   Purpose

   BromoTag-mClover for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-BromoTag-mClover
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK447 (BSD-P2A-BromoTag-mCherry)

   Plasmid

   #214377

   Purpose

   BromoTag-mCherry for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BSD-P2A-BromoTag-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-T3-SpCAS9-NG-pA

   Plasmid

   #131465

   Purpose

   Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized SpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV Puro-pGK:GFP

   Plasmid

   #68486

   Purpose

   Retrovirus expressing GFP driven by pGK promoter

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Puromycin Resistance
   GFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR and pGK

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. A3Bi-Cas9n-UGI

   Plasmid

   #119135

   Purpose

   Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3B L1 intron-Cas9 nickase-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   L1 intron insertion

   Promoter

   CMV

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET23a-HsUBB

   Plasmid

   #69562

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCLX-M38s/TOP-dGFP

   Plasmid

   #114295

   Purpose

   Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   dGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   destabilized GFP

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEFS-ccdB-12X MS2_mPGK-Puro

   Plasmid

   #177809

   Purpose

   Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

   Type

   Empty backbone

   Use

   Tags

   12X MS2 stem loops

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-EcPPK1

   Plasmid

   #159279

   Purpose

   Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

   Depositor

   Michael Downey

   Article

   Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

   Insert

   HA-tagged Polyphosphate Kinase 1
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK282 (mCherry2-Bsr)

    Plasmid

    #72798

    Purpose

    C-terminal tagging with mCherry2 using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mCherry2-Bsr
    

    Use

    CRISPR

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. TOPO pTRE

    Plasmid

    #68457

    Purpose

    TOPO construct expressing pTRE for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Tetracycline Response Element Promoter
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTubulin NLS-mCherry-LEXY

    Plasmid

    #182597

    Purpose

    Drosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    mCherry
    

    Use

    Tags

    LEXY and NLS

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CIBN-CreC

    Plasmid

    #75267

    Purpose

    The first half of the photo-activatable cre recombinase

    Depositor

    Michael Bruchas

    Article

    Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

    Insert

    N-terminal portion of the CIB1 transcription factor
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRE:shRNA A-pGK:rtTA-Cre

    Plasmid

    #68471

    Purpose

    Inducible lentivirus expressing an arbitrary shRNA and Cre

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Inserts

    GFP-shRNA A-miR30
    rtTA-2A-Cre
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK and pTRE

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMBA323

    Plasmid

    #214751

    Purpose

    To create delta(glmS,nadE) working strains and maintaining their viability

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CT3G-ERP2

    Plasmid

    #175497

    Purpose

    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available since

    Oct. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2-ngn1-Trpa1b-2A-mCherry

    Plasmid

    #106425

    Purpose

    For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Inserts

    cmlc2 promoter
    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pMXs-PT2A-GFP-iRFP670-Tdtomato

    Plasmid

    #111813

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    GFP
    iRFP670
    Tdtomato
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. miR-vector-RFP pCDNA

    Plasmid

    #97143

    Purpose

    for expression of miRNA and TagRFP

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE Zeo IkB SR

    Plasmid

    #83023

    Purpose

    Retroviral expression of dominant negative IkB alpha

    Depositor

    Yi Zhang

    Article

    Kim et al Cell Rep. 2016 Sep 27;17(1):275-288. doi

    Insert

    NFKB Inhibitor Alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Serine 32 to Alanine, Serine 36 to Alanine

    Promoter

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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