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pNK5712

Plasmid

#219752

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Publication

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnH3H_v2 - tAOX

Expression

Yeast

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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A2C-∆N

Plasmid

#217909

Purpose

Expresses bicone gas vesicles in E. coli

Depositor

Mikhail Shapiro

Publication

Ling et al Adv Mater. 2024 Feb 26:e2307106. doi: 10

Insert

A2C-∆N

Expression

Bacterial

Promoter

T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-luxOFP

Plasmid

#229945

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Publication

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-HA-deLACCO1-NGR

Plasmid

#208097

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Publication

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

deLACCO1

Use

AAV

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-WHaloCaMP1a

Plasmid

#205312

Purpose

Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Insert

WHaloCaMP1a

Use

Zebrafish expression

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-hSyn-jGCaMP7b-XC

Plasmid

#182221

Purpose

Improved genetic encoded calcium sensor based on GCaMP7b.

Depositor

Xiaodong Liu

Publication

Geng et al Elife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XC

Use

AAV

Expression

Mammalian

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_354

Plasmid

#180525

Purpose

KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-Ceru-10unit

Plasmid

#158389

Purpose

10-unit intMEMOIR recording array for Drosophila melanogaster

Depositor

Michael Elowitz

Publication

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

10unit recording array

Expression

Insect

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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AL12R

Plasmid

#161007

Purpose

PP7-tagged reporter without a promoter

Depositor

Krishna Niyogi

Publication

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Type

Empty backbone

Tags

PP7 and H2B-mScarlet

Expression

Plant

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-mCerulean

Plasmid

#66084

Purpose

G protein alpha-s internally tagged with mCerulean and EE epitope

Depositor

Catherine Berlot

Publication

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

G-alpha-s-EE-mCerulean (Gnas Rat, Aequorea victoria)

Tags

internal EE epitope (residues 189-194 in alpha-s …

Expression

Mammalian

Mutation

BamHI sites in the alpha-s cDNA were removed and …

Promoter

CMV

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pNK2657

Plasmid

#219741

Purpose

MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

Depositor

Alexander Mishin

Publication

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

hispidin- synthase from Mycena citricolor

Use

Synthetic Biology

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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BSP605

Plasmid

#122250

Purpose

expression vector for mCerulean under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mCerulean

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hVoS 1.5 thy-1

Plasmid

#45281

Depositor

Meyer B. Jackson

Publication

Wang et al Biophys J. 2010 Oct 6;99(7):2355-65. doi

Insert

CeFP

Use

Targeting

Tags

truncated h-ras motif

Promoter

thy1

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
14-3-3 KO vector

Plasmid

#16551

Depositor

Bert Vogelstein

Publication

Chan et al Nature. 1999 Oct 7. 401(6753):616-20.

Insert

14-3-3 knockout (SFN Human)

Expression

Bacterial

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
Immunostealth

Plasmid

#239446

Purpose

Expresses murine NIS (mNIS) in mammalian cells for non-immunogenic in vivo imaging using SPECT or PET

Depositor

Scott Lyons

Publication

Merrill et al Cell Stress. 2023 Aug 14;7(8):59-68. doi

Insert

sodium iodide symporter (Slc5a5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-elavl3-WHaloCaMP1a-EGFP

Plasmid

#205311

Purpose

Expression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)

Depositor

Eric Schreiter

Publication

Farrants et al Nat Methods. 2024 Oct;21(10):1916-1925.

Insert

WHaloCaMP1a-EGFP

Use

Zebrafish expression

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAV-U6+27-Tornado-biRhoBAST

Plasmid

#196344

Purpose

Overexpression of circular biRhoBAST

Depositor

Murat Sunbul

Publication

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

Tornado-biRhoBAST

Expression

Mammalian

Promoter

U6+27

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLBS.CAG-pHlu

Plasmid

#129331

Purpose

pH measurement, lentivirus

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

pHluorin (from H. Imamura)

Use

Lentiviral

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAG469-LifeAct-redFAST

Plasmid

#160365

Purpose

Expresses LifeAct-redFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

LifeAct-RedFAST

Expression

Mammalian

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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