We narrowed to 10,914 results for: CHL

1. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

   Plasmid

   #208957

   Purpose

   A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. HUHe variant click editor (CE1) - pCMV-T7-MSMV-nCas9-EcKlenow (JO661)

   Plasmid

   #208947

   Purpose

   A variant CE1 construct with MSMV HUH endonuclease, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   MSMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

   Plasmid

   #179436

   Purpose

   Dual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   HS4-TRE-cit-HS4-mch (DH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha, pRSV

   Available since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29 (JO582)

   Plasmid

   #208954

   Purpose

   A variant CE1 construct with Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-Phi29(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A), Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKK-RNAi-nucEGFPmiR-TEV-mCherry

   Plasmid

   #105812

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with C-terminal mCherry.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR v2 puro - CRBN (#2)

   Plasmid

   #166241

   Purpose

   sgRNA (#2) to generate a knockout of human CRBN by targeting the start region of Exon1

   Depositor

   Günter Schneider

   Article

   Lier et al Bioorg Chem. 2021 Nov 20:105505. doi: 10

   Insert

   CRBN (CRBN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet-O-FUW-NR4A3-P2A-mCherry

   Plasmid

   #203863

   Purpose

   Tet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   NR4A3 (NR4A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tet-O-FUW-NR4A1-P2A-mCherry

   Plasmid

   #203859

   Purpose

   Tet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   NR4A1 (NR4A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHOT-P2Y2R

   Plasmid

   #159108

   Purpose

   entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

   Depositor

   Fabio Mammano

   Article

   Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

   Insert

   P2Y2R
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UBC

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. plentiCRISPRv2-PURO-LYSET-gRNA1

    Plasmid

    #210489

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA1 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. plentiCRISPRv2-PURO-LYSET-gRNA2

    Plasmid

    #210490

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA2 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid

    #137885

    Purpose

    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Inserts

    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-RNAi-nucCHERRYmiR-EGFP-TEV

    Plasmid

    #105810

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal EGFP.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    EGFP-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKK-RNAi-nucCHERRYmiR-FLAG-TEV

    Plasmid

    #105809

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    FLAG-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. BAP1-001

    Plasmid

    #68365

    Purpose

    stable expression of BAP1 in mammalian cells

    Depositor

    Emanuela Felley-Bosco, Rolf Stahel

    Article

    Parrotta et al J Thorac Oncol. 2017 Apr 4. pii: S1556-0

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

16. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid

    #217798

    Purpose

    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR and Lentiviral

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    EF1a

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid

    #208943

    Purpose

    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid

    #179437

    Purpose

    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    coreHS4-TRE-cit-coreHS4-mch (DC)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTriEx-PA-Rac1-C450A

    Plasmid

    #22025

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains light-insensitive mutation C4…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pTriEx-PA-Rac1-I539E

    Plasmid

    #22026

    Purpose

    Expression of photoactivatable Rac1 ('lit' state mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-I539E (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV domain contains 'lit state' mutatio…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only