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Showing: 3041 - 3060 of 22473 results

  4. His8:MBP-tev-Ala-eK-Flv

    Plasmid
    #83393
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83393-plasmid-map-sequence-id-174595

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  5. His8:MBP-tev-Ile-eK-Flv

    Plasmid
    #83392
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83392-plasmid-map-sequence-id-174594

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  6. His8:MBP-tev-Tyr-eK-Flv

    Plasmid
    #83391
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83391-plasmid-map-sequence-id-174593

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  7. p.UTA.2.0-GL-2-3 perfect copies

    Plasmid
    #82441
    Purpose
    Dual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2
    Depositor
    Jens Gruber
    Article
    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10
    Insert
    Luciferase
    Use
    Tags
    Expression
    Mutation
    The complementary region for GL2 siRNA control wa…
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82441-plasmid-map-sequence-id-162054

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  10. pET22-HT/purC

    Plasmid
    #73623
    Purpose
    Produces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tag
    Depositor
    James Williamson, T. J. Kappock
    Article
    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.
    Insert
    phosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Contains multiple silent mutations (E179, G185, G…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73623-plasmid-map-sequence-id-136194

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  14. pJK048

    Plasmid
    #75230
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Article
    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and n/a
    Available since
    Availability
    Academic Institutions and Nonprofits only
    75230-plasmid-map-sequence-id-142240

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  15. pDD2000

    Plasmid
    #101197
    Purpose
    Name: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.
    Depositor
    Dirk Dittmer
    Article
    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1
    Insert
    LANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    101197-plasmid-map-sequence-id-194526

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  16. pcDNA3-PKAsub(T/A)-RLuc8N-NES

    Plasmid
    #138212
    Purpose
    Encodes negative-control substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub(T/A)-RLuc8N-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138212-plasmid-map-sequence-id-307414

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  20. pcDNA3-Clover

    Plasmid
    #40259
    Purpose
    GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    Clover GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE
    Available since
    Sept. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    40259-plasmid-map-sequence-id-215680

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Showing: 3041 - 3060 of 22473 results