We narrowed to 9,861 results for: SUB;

1. pETDuet RagA-RagC(D181N)

   Plasmid

   #99643

   Purpose

   Bacterial expression of RagA-RagC(D181N)

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D181N

   Promoter

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-GFP-SFPQ-RT

   Plasmid

   #97090

   Purpose

   Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   EGFP-SFPQ-RT (SFPQ Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCSG28-SH3-588

   Plasmid

   #185920

   Purpose

   Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

   Depositor

   Brian Kuhlman

   Article

   Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

   Insert

   SH3-588
   

   Use

   Tags

   6x HIS tag

   Expression

   Bacterial

   Mutation

   Designed enzyme with novel interface between huma…

   Promoter

   T7

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. optoPKAW196R/Y204A

   Plasmid

   #104546

   Purpose

   Expresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Cry2-mCherry-PKA W196R/Y204A
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   PKA W196R and Y204A

   Promoter

   CMV

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-M2-PAK2 wt

   Plasmid

   #31680

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   p21-activated protein kinase 2 (PAK2 Human)
   

   Use

   Lentiviral

   Tags

   M2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH6_new

   Plasmid

   #105866

   Purpose

   more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

   Depositor

   Andreas Weber

   Article

   Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

   Insert

   U6-26p::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Subcloning

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-CenpB 1-166

   Plasmid

   #108475

   Purpose

   expression of GFP-CenpB 1-166

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CenpB 1-166 AA (CENPB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   encodes only the DNA-binding domain of CENP-B

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC_C_TGON2

   Plasmid

   #111677

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   TGON2 (TGOLN2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSunS

   Plasmid

   #171672

   Purpose

   SunS expression in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

   Insert

   Glycosyltransferase SunS (sunS Bacillus subtilis (strain 168))
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICH-nuc-roGFP2-GPXC71S

    Plasmid

    #191760

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    GPX has no peroxidatic Cysteine 71, which is repl…

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4136464

    Plasmid

    #149534

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pOBCol3.6-GFPtpz

    Plasmid

    #110211

    Purpose

    Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

    Depositor

    David Rowe

    Article

    Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

    Insert

    eGFPtopaz
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    3.6 kb rat type 1 collagen + first intron

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILGFPH7

    Plasmid

    #83554

    Purpose

    Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    ADH2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH2

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEAK-bKal7

    Plasmid

    #145800

    Purpose

    expresses Kalirin-7 with the b-front in mammalian cells

    Depositor

    Betty Eipper, Richard Mains

    Article

    Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

    Insert

    bKalirin-7 (Kalrn Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EIF2a

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCX-Lyn-SponGee

    Plasmid

    #134776

    Purpose

    mammalian expression of Lyn-SponGee, a lipid raft-targeted variant of the cGMP buffer SponGee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

    Insert

    Lyn-SponGee
    

    Use

    Tags

    FLAG and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-HisD-KRaION1

    Plasmid

    #177799

    Purpose

    Bacterial expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4046799

    Plasmid

    #145577

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. SEPTOPO

    Plasmid

    #64944

    Purpose

    source of SEP (Super Ecliptic pHluorin) sequence

    Depositor

    Jeremy Henley

    Article

    Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

    Insert

    SEP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGBW-m4136461

    Plasmid

    #149538

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Venus wGBD

    Plasmid

    #85222

    Purpose

    Venus tagged Cdc-42 sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Insert

    wGBD (WASL Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Cdc42 binding domain of N-WASP

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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