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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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U6::sgBFP-U6::sgKrasG12D-pX333-red

Plasmid

#149448

Purpose

pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

Depositor

Jonathan Chernoff

Article

Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

Insert

sgRNA BFP and mouse KRAS G12D

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pOZ-Pax3-FH-C-puro

Plasmid

#32517

Depositor

Andrew Lassar

Article

Kumar et al Mol Biol Cell. 2009 Jul;20(14):3170-7. E

Insert

Pax3 (Pax3 Mouse)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
TAL2384

Plasmid

#36793

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BRCA1-TALEN_Left (Brca1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pLenti_TMv2

Plasmid

#131761

Purpose

frameshift reporter for barcode retrieval

Depositor

Paul Blainey

Article

Feldman et al BMC Biol. 2020 Nov 24;18(1):177. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-NucGFP

Plasmid

#184696

Purpose

Lentiviral transfer plasmid for expression of NucGFP

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

NucGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate5

Plasmid

#164999

Purpose

Expression of candidate TCR #5 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate5

Plasmid

#165000

Purpose

Expression of candidate TCR #5 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1.mir451_KO

Plasmid

#164689

Purpose

For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-451 CRISPR KO gRNA (MIR451A )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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AAV.pCry1(408).DIO.Luciferase

Plasmid

#192623

Purpose

Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

Depositor

Michael Hastings

Article

Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

Insert

luciferase

Use

AAV

Expression

Mammalian

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304 LacZ-V5 blast

Plasmid

#83099

Purpose

Lentiviral vector for constitutive expression of LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
Dopey2_pCSdest

Plasmid

#53850

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

Dopey2

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
LoriT

Plasmid

#163691

Purpose

Modified L4440 containing now CamR and oriT for conjugation

Depositor

Baris Tursun

Article

Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

Insert

oriT

Expression

Bacterial

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAG260

Plasmid

#33210

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

SYNM (SYNM Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
PRMT2_pCSdest

Plasmid

#53905

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PRMT2 (PRMT2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
RBM11_pCSdest

Plasmid

#53861

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RBM11 (RBM11 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
CRISPR_TRBC

Plasmid

#164994

Purpose

Expression of gRNA targeting TCRbeta constant locus

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against TRBC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate7

Plasmid

#165004

Purpose

Expression of candidate TCR #7 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate7

Plasmid

#165003

Purpose

Expression of candidate TCR #7 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;clecl14aCE1-P1Egfp

Plasmid

#90157

Purpose

contains clec14a enhancer sequence, and clec14a specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1P1Egfp

Tags

EGFP

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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