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We narrowed to 14,310 results for: eng

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Showing: 3041 - 3060 of 14310 results

  1. dCas9-10xSunTag-P2A-tagBFP

    Plasmid
    #174141
    Purpose
    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.
    Depositor
    Albert Jeltsch
    Publication
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Insert
    dCas9, 10xSunTag, tagBFP
    Tags
    HA and tagBFP
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    174141-plasmid-map-sequence-id-345615

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  7. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    65768-plasmid-map-sequence-id-112655

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  8. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Publication
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    154840-plasmid-map-sequence-id-307910

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  18. pRVL-3

    Plasmid
    #104581
    Purpose
    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Publication
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and E318A/K320A/K322A
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    104581-plasmid-map-sequence-id-201392

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Showing: 3041 - 3060 of 14310 results