We narrowed to 6,032 results for: plasmid dna

1. SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

   Plasmid

   #169283

   Purpose

   Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

   Depositor

   Dirk Heckl

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. cTRE-LSL-GFP-IRES-Cas9-sgCR8

   Plasmid

   #135668

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgCR8
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

   Plasmid

   #135669

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgPten
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEFL

   Plasmid

   #38121

   Depositor

   JS Gutkind

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInt-ERCreER

   Plasmid

   #190040

   Purpose

   This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

   Depositor

   Kayoko Tanaka

   Article

   Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

   Insert

   ERT2-Cre-ERT2 flanked by AAVS1
   

   Use

   CRISPR and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRN3P_T3_ABE8e_IVT

   Plasmid

   #201676

   Purpose

   Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

   Insert

   ecTadA(8e)-nSpCas9
   

   Use

   CRISPR; Vector for in-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T3 promoter

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

   Plasmid

   #121833

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/Dnmt3a-3L (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR-Cas9/CD4-TK2

   Plasmid

   #104397

   Purpose

   The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

   Depositor

   Jeronimo Blanco

   Article

   Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

   Insert

   TK2 (TK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

   Plasmid

   #121826

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9/Dnmt3a-3L (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

    Plasmid

    #182894

    Purpose

    Mammalian expression of PVALB rat mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Slack Slo2.2 max1-ECFP

    Plasmid

    #197318

    Purpose

    Plasmid expressing an antigen used to generate an antibody that targets the Slack K+ channel subunit

    Depositor

    Christopher Lingle

    Insert

    KCNT1/Slo2.2/Slack K+ channel (Kcnt1 Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Nemato

    Plasmid

    #24006

    Depositor

    Lawrence Cohen

    Insert

    VSFP
    

    Use

    Tags

    Cerulean and Citruline

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOME0294M

    Plasmid

    #182623

    Purpose

    KSHV anti-RTA_3Kb_Reverse

    Depositor

    Dirk Dittmer

    Insert

    KSHV anti-RTA_3Kb_Reverse
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion at 2853

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-noPS

    Plasmid

    #177943

    Purpose

    Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Luc-noPS

    Plasmid

    #177940

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. L-SACSIN-74

    Plasmid

    #166526

    Purpose

    scFv of a human scaffold targeting Sacsin, DnaJ homolog subfamily C member 29. Antigen coverage aa 84-339 of 4579

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOME0295M

    Plasmid

    #182624

    Purpose

    KSHV Reverse Sequence Negative control Kaposinclouster_RevCompl-KapATGdel_Syn

    Depositor

    Dirk Dittmer

    Insert

    KSHV Kaposincluster_RevCompl_KapATGdel_SYN_rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid

    #182895

    Purpose

    Mammalian expression of PVALB rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid

    #74408

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid

    #163643

    Purpose

    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    

    Use

    Lentiviral

    Tags

    Luc2-P2A-tdTomato-T2A

    Expression

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    EFS and pU6

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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