We narrowed to 6,903 results for: plasmid dna

1. pFA0057

   Plasmid

   #131784

   Purpose

   Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

   Depositor

   Frank Albert

   Article

   Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

   Insert

   20mer GFP cassette guide (gGFP) and 5' sgRNA
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AY23_pgRNA.R5.2

   Plasmid

   #100295

   Purpose

   CCR5-targeting gRNA expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 Polymerase III snRNA

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWN10042

   Plasmid

   #89052

   Purpose

   GF-ori-FP plasmid for GFxFP assay

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   EGFP part 1
   EGFP part 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and none

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDGB1alpha1_SF (GB0106)

   Plasmid

   #68232

   Purpose

   Twister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Solanum lycopersicum intergenic region
   

   Use

   Synthetic Biology; Unspecified

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDGB1omega1_SF (GB0158)

   Plasmid

   #68242

   Purpose

   Twister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Solanum lycopersicum intergenic region
   

   Use

   Synthetic Biology; Unspecified

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2

   Plasmid

   #69228

   Purpose

   Expresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS2 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3

   Plasmid

   #69229

   Purpose

   Expresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS3 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4

   Plasmid

   #69230

   Purpose

   Expresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS4 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTO128_pPZP-NATca

   Plasmid

   #171105

   Purpose

   A. tumefaciens TI plasmid encoding NATca in T-DNA

   Depositor

   Teresa O'Meara

   Article

   Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

   Insert

   NAT (Nourseothricin Resistance)
   

   Use

   A. tumefaciens ti plasmid

   Tags

   Expression

   Mutation

   Promoter

   AgTEF2

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA

    Plasmid

    #89460

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Use

    Tags

    hinge + HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA

    Plasmid

    #89459

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DNMT3B (3QKJ)

    Plasmid

    #32044

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DNMT3B (DNMT3B Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    PWWP domain only (amino acids 293-442)

    Promoter

    Available Since

    Feb. 22, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pFLAG-CMV4-GAL4 DBD-Aire CTT

    Plasmid

    #241399

    Purpose

    Mammalian gene expression vector for GAL4 DNA binding domain fused to Aire C-terminal tail (aa 491-552)

    Depositor

    Sun Hur

    Insert

    Gal4-DBD Aire C-terminal tail (AIRE Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Truncated Aire; contains aa 491-552

    Promoter

    CMV

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p249_LTJ_1kbFoxp3wtTemplate

    Plasmid

    #82664

    Purpose

    1kb HDR template for murine wildtype Foxp3

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    1kb genomic DNA sequence from murine wildtype Foxp3
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p250_LTJ_2kbFoxp3wtTemplate

    Plasmid

    #82665

    Purpose

    2kb HDR template for murine wildtype Foxp3

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    2kb genomic DNA sequence from murine wildtype Foxp3
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQLL-MAG-5 kb

    Plasmid

    #218305

    Purpose

    Donor plasmid of MAG Transposon with 5 kb cargo gene

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    5 kb cargo gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-i53

    Plasmid

    #92170

    Purpose

    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    i53
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    UbvG08 with I44A mutation and no terminal Glycines

    Promoter

    CMV

    Available Since

    July 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pICOZ-SB100X[EF1α-CD19 4-1BB]

    Plasmid

    #218328

    Purpose

    Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUW-[EF1α-CD19 4-1BB]

    Plasmid

    #218336

    Purpose

    Lentiviral transfer plasmids for mammalian expression CD19 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUW-[EF1α-CD19 CD28Z]

    Plasmid

    #218338

    Purpose

    Lentiviral transfer plasmids for mammalian expression CD19 CAR

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge