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KJ201: pMVP (R4-R3) osTir1 open

Plasmid

#121743

Purpose

pMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

osTir1 (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

9x myc epitope tag

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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C21orf2_pCSdest

Plasmid

#53911

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

C21orf2 (C21orf2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pTC440

Plasmid

#91225

Purpose

protoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting barley MLO

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTC441

Plasmid

#91218

Purpose

protoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting MLO gene in barley

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTC436

Plasmid

#91222

Purpose

protoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNA targeting barley MLO

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTC437

Plasmid

#91219

Purpose

protoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting MLO gene in barley

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 miR 19b-3p

Plasmid

#82442

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-19-3p

Mutation

Complementary region for hsa-miR-19-3p as sensor

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos DD F343A

Plasmid

#8972

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D, F343A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos DD Y345A

Plasmid

#8971

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362D, S374D, Y345A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pICSL80037

Plasmid

#68260

Purpose

Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, neomycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11061

Plasmid

#68255

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-1

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-FOXA1

Plasmid

#139304

Purpose

Knocking down of human FOXA1 through shRNA and amiRNA targeting FOXA1

Depositor

Claes Wadelius

Article

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against FOXA1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND4_L17_S24_S11_S19_S13

Plasmid

#132732

Purpose

T7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND4_L17_S24_S11_S19_S13

Expression

Bacterial

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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ARB287d

Plasmid

#124018

Purpose

Encodes pEF1a driving expression of mAzamiGreen with a spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-spacer_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB287e

Plasmid

#124019

Purpose

Encodes pEF1a driving expression of mAzamiGreen with a multicistronic spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-spacermulticistronic_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0465

Plasmid

#124036

Purpose

Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of a mScarlet-targeting sgRNA in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pEF1a-iRFP713_mScarlet sgRNA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0468

Plasmid

#124038

Purpose

Encodes pEF1a driving expression of nuclear iRFP713, and mu6 expression of tagBFP- and mScarlet-targeting sgRNAs in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pEF1a-iRFP713_mScarletsgRNA_tagBFP sgRNA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB287a

Plasmid

#124015

Purpose

Encodes pEF1a driving expression of mAzamiGreen with a BgpPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB287b

Plasmid

#124016

Purpose

Encodes pEF1a driving expression of mAzamiGreen with a SV40 3' UTR and a mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-SV40pA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB287c

Plasmid

#124017

Purpose

Encodes pEF1a driving expression of mAzamiGreen with a RglPA 3' UTR and a mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-rglpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAG_Ptet*_ToeRep_N01_switch

Plasmid

#132737

Purpose

Inducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_ToeRep_N01_switch

Expression

Bacterial

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_Ptet*_3WJrep_N19_switch

Plasmid

#132739

Purpose

Inducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoter

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

Ptet*_3WJrep_N19_switch

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pYZ_NAND3_L11_S11_S13_S19

Plasmid

#132731

Purpose

T7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND3_L11_S11_S13_S19

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjPLP1e2-A39T

Plasmid

#129752

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

PLP1 (PLP1 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

A39T

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjPLP1e2-P15L

Plasmid

#129753

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

PLP1 (PLP1 C. jacchus (common marmost))

Use

Marmoset targeting

Mutation

P15L

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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JK305: pMVP/Blast-DEST

Plasmid

#121855

Purpose

pMVP destination vector, empty expression vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JL104: pMVP/Neo-DEST

Plasmid

#121857

Purpose

pMVP destination vector, empty expression vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS

Plasmid

#121827

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-Cas9 (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY001: pMVP (L3-L2) BioID2 + polyA

Plasmid

#121795

Purpose

pMVP L3-L2 entry plasmid, contains BioID2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

BioID2 + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY202: pMVP (L3-L2) APEX2 + polyA

Plasmid

#121796

Purpose

pMVP L3-L2 entry plasmid, contains APEX2 + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term APEX2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

APEX2 + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge