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Plasmid#86035Purposehuman VEGFR1 with a 10 amino acid deletion in EC close to TM domainDepositorInserthuman Flt1 delta754-763 aa (FLT1 Human)
UseTagsFLAG, HA, and MycExpressionMammalianMutationdelta 754-763PromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
ECM2-bio-His
Plasmid#53425PurposeExpresses full-length Extracellular matrix protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertECM2 (ECM2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(K68A,K69A,F70A,F71A) in pcDNA3.1
Plasmid#42189DepositorInsertGamma 3(K68A,K69A,F70A,F71A) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationK68A,K69A,F70A,F71APromoterCMVAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(A73L,L74I,L75S) in pcDNA3.1
Plasmid#42190DepositorInsertG protein Gamma 3(A73L,L74I,L75S) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationA73L,L74I,L75SPromoterCMVAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE
Plasmid#228766Purposeinsert a blue fluorescent protein mBFP2, a cyclin-dependent kinase inhibitor p27KDepositorInsertp27 kinase inhibitor (Cdkn1b Mouse)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pStreptagII 3C SMO
Plasmid#234995PurposeExpression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptorDepositorInsertSMO (SMO Human)
UseTagsStreptag-II, HRV 3C siteExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNA 3C myc scfvFLAG
Plasmid#234990PurposeExpression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteinDepositorInsertNA-3C-myc-scfvFLAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-AHPH
Plasmid#187278PurposeExpress mCherry-AHPH (RhoA biosensor)DepositorInsertAnillin and Pleckstrin homology domain (ANLN Human)
UseTagsmCherryExpressionMammalianMutationAHPHPromoterCMVAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
IL2-mCherry in pmCherryN1
Plasmid#187283PurposeExpress IL2-mCherryDepositorInsertinterleukin-2 receptor alpha-subunit (IL2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR
Plasmid#201539PurposeLanding pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)DepositorInsertsPuromycinR-P2A-miRFP720
ZF6x12-C-DsRed-Express2(x6)
BlasticidinR
UseTagsExpressionMammalianMutationPromoterNone, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…Available sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-TGFa-ScNeo
Plasmid#209904PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
UseTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-TGFa-ScNeo
Plasmid#209908PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
UseTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
p50.mALDH1A1.CD63-GFP.wpre
Plasmid#226770PurposeCD63-GFP in p52 (mAldh1a backbone)DepositorInsertCD63 (CD63 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
DG SaCas9 GFP V3
Plasmid#226961PurposeCBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDG459 V3
Plasmid#226958PurposeCBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
SaCas9 GFP V3
Plasmid#226962PurposeCBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pdg459-eSp(1.1) V3
Plasmid#226965PurposeCBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
DG SaCas9 puro V3
Plasmid#226960PurposeCBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDG458 eSp(1.1) V3
Plasmid#226964PurposeCBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRKCZ-C20-EGFP (C-PRKCZ-C20)
Plasmid#217761PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertProtein kinase C zeta (PRKCZ Human)
UseTagsEGFPExpressionMammalianMutationC20 domain (aa 405-592)PromoterCMVAvailable sinceJune 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits