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MO70 - Orai1 EC-Flag

Plasmid

#79590

Purpose

Transient expression and retroviral Expression

Depositor

Yousang Gwack

Article

Srikanth et al J Biol Chem. 2010 Feb 12;285(7):5066-75.

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

FLAG (inserted between TM3 and TM4 of the protein…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
Sema3f(L,+)-Fc-His

Plasmid

#72147

Purpose

Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3a(L, del3)-AP-His

Plasmid

#72010

Purpose

Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-MKL1 D301-380

Plasmid

#19852

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 301 to 380

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

Plasmid

#163644

Purpose

Makes a lentivirus that expresses mNeonGreen and Cas9.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

mNeonGreen-P2A-Cas9

Use

Lentiviral

Tags

mNeonGreen-P2A

Promoter

EFS

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-Squ5'3'

Plasmid

#20165

Depositor

Eric Wieschaus

Article

Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

Insert

spaghetti squash (squ, myosin II RLC) 5' UTR, ORF (without stop codon) and 3' UTR with multiple cloning site downstream (sqh Fly)

Expression

Bacterial

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
sTR056

Plasmid

#177369

Purpose

Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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sTR060

Plasmid

#177370

Purpose

Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

Trigger RNA

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

Plasmid

#174642

Purpose

Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

Tags

SSPB(micro)-Venus

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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Sema3a(S)-AP-His

Plasmid

#72012

Purpose

Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3b(L)-AP-His

Plasmid

#72013

Purpose

Expresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3b (Sema3b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 D444-500

Plasmid

#19839

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D444-500 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 444-500

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

Plasmid

#107728

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Plasmid

#173865

Purpose

Mammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 T450A

Plasmid

#19843

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 T450A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Threonine 450 to Alanine

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO myc p31Comet

Plasmid

#59833

Purpose

Allows the integration of myc p31Comet in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Westhorpe et al J Cell Sci. 2011 Nov 15;124(Pt 22):3905-

Insert

p31 (MAD2L1BP Human)

Tags

Myc

Expression

Mammalian

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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dmBACCS2VL-IRES-dOrai-IRES-GFP

Plasmid

#72897

Purpose

Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

dmBACCS2VL-IRES-dOrai-IRES-GFP

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 D500-630

Plasmid

#19841

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D500-630 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 500-630

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
p3xFLAG-MKL1 D381-506

Plasmid

#19853

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 381 to 506

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA 3.3 HA-TSPAN12^11-LEL

Plasmid

#115785

Purpose

Expresses a chimera in mammalian cells: the large extracellular loop of TSPAN12 is replaced with TSPAN11 sequence

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

TSPAN12/11 chimera (TSPAN11, TSPAN12 Human)

Tags

HA

Expression

Mammalian

Mutation

large extracellular loop of TSPAN12 replaced with…

Promoter

CMV

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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