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We narrowed to 11,025 results for: gal

Showing: 7701 - 7750 of 11025 results

  2. EcNGsC-DelFSH(307-309)-pKK223

    Plasmid
    #131377
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.
    Depositor
    Martin Horvath
    Article
    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021
    Insert
    EcNGsC MutY chimera DelFSH(307-309)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131377-plasmid-map-sequence-id-259231

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  3. EcNGsC-S308P-H309D-pKK223

    Plasmid
    #131378
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.
    Depositor
    Martin Horvath
    Article
    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021
    Insert
    EcNGsC MutY chimera S308P H309D
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are from…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131378-plasmid-map-sequence-id-259242

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  4. EcNGsC-F307A-S308A-pKK223

    Plasmid
    #131379
    Purpose
    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes F307A and S308A in Gs MutY.
    Depositor
    Martin Horvath
    Article
    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021
    Insert
    EcNGsC MutY chimera F307A S308A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Fusion of two MutY genes. Residues 1-225 are fro…
    Promoter
    tac
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131379-plasmid-map-sequence-id-259243

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  5. pABD823

    Plasmid
    #67911
    Purpose
    Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    Cold temperature germinating 10 (CTG10)
    Use
    Tags
    GAL4 DNA binding domain (148 amino acids)
    Expression
    Yeast
    Mutation
    Promoter
    pADH1 (alcohol dehydrogenase 1 promoter)
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  24. pCDH-SFFV-GLuc-CreERT2-mCherry

    Plasmid
    #178185
    Purpose
    the plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.
    Depositor
    Irmela Jeremias
    Article
    Carlet et al Nat Commun. 2021 Sep 27;12(1):5655. doi:
    Inserts
    GLuc
    CreERT2
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    178185-plasmid-map-sequence-id-351314

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  26. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Use
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49169-plasmid-map-sequence-id-72495

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  28. pLenti-nSpCas9-TadA8e

    Plasmid
    #209044
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    TadA8e-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209044-plasmid-map-sequence-id-413636

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  33. pLCHKOv3-mCherry-intergenic-As

    Plasmid
    #209037
    Purpose
    Lentiviral vector expressing mCherry along with U6 driven hybrid guide (hgRNA) targeting two intergenic sites in the human genome using SpCas9 and AsCas12a nucleases (CHyMErA system), respectively
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    intergenic hgRNA with SpCas9 tracrRNAv1 & AsCas12a DR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209037-plasmid-map-sequence-id-413702

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  34. iMb2-Control-Mosaic (SO273)

    Plasmid
    #99750
    Purpose
    Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins
    Depositor
    Rui Benedito
    Article
    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do
    Insert
    Mb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99750-plasmid-map-sequence-id-443585

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  39. pTT5-hSDC1-CS1/CS2

    Plasmid
    #52370
    Purpose
    expresses human Syndecan-1 point mutant at S184A and S194A in HEK293-EBNA1 (293E) suspension culture.
    Depositor
    Gordon Laurie
    Article
    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1
    Insert
    SDC1 (SDC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R95Q, S184A and S194A (according to numbering in …
    Promoter
    CMV
    Available since
    Dec. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  45. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid
    #194190
    Purpose
    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    5 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194190-plasmid-map-sequence-id-381947

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  46. pGL3-pSMANTIS_1kb_KLF2mut 899

    Plasmid
    #194193
    Purpose
    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)
    Depositor
    Matthias Leisegang, Ralf Brandes
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated KLF binding sites (position 899)
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194193-plasmid-map-sequence-id-381931

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  48. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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Showing: 7701 - 7750 of 11025 results