Addgene

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pAD120-CHIP-G132N-myc-His

Plasmid

#236053

Purpose

Mammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

CHIP-G132N (STUB1 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

G132N

Promoter

CMV

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAD000-CHIP-K30A-myc-His

Plasmid

#236052

Purpose

Mammalian expression of human CHIP with the synthetic K30A substitution and C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

CHIP-K30A (STUB1 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

K30A

Promoter

CMV

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 StoG

Plasmid

#226610

Purpose

All serines in the LC domain to glycine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 StoG (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All serines to glycine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 TtoS

Plasmid

#226611

Purpose

All threonines in the LC domain to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 TtoS (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All threonine to serine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoA

Plasmid

#226612

Purpose

All glutamines in the LC domain to alanine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoA (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All glutamine to alanine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 TtoV

Plasmid

#226613

Purpose

All threonines in the LC domain to valine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 TtoV (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All threonine to valine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoG

Plasmid

#226614

Purpose

All glutamines in the LC domain to glycine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoG (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All glutamine to glycine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoN

Plasmid

#226615

Purpose

All glutamines in the LC domain to asparagine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoN (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All glutamine to asparagine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoS

Plasmid

#226616

Purpose

All glutamines in the LC domain to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoS (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

All glutamine to serine in the LC domain

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC noYQ_noYT

Plasmid

#226617

Purpose

T7S / Q160S double mutation

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC noYQ_noYT (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

T7S / Q160S double mutation in LC domain to remov…

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 wild type

Plasmid

#226596

Purpose

Wild type sequence of FUS LC-RGG1 domains

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 WT (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

wild type LC-RGG1

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->AA

Plasmid

#226600

Purpose

4 glutamine pairs to alanine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->AA (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with AA pairs at positions 27…

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->GG

Plasmid

#226601

Purpose

4 glutamine pairs to glycine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->GG (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with GG pairs at positions 27…

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4QQ->NN

Plasmid

#226602

Purpose

4 glutamine pairs to asparagine pairs at positions 27, 28, 92, 93, 132, 133, 157, 158

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->NN (FUS Human)

Use

Tags

6His

Expression

Bacterial

Mutation

4 glutamine pairs to asparagine pairs at position…

Promoter

T7

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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6xHsa.NFKB:Kaede

Plasmid

#239992

Purpose

Kaede driven by NFkB activity can be switched from green to red fluorescence by UV light exposure, enabling tracking of NFkB positive cells

Depositor

Raquel Espin-Palazon

Insert

photo convertible Kaede

Use

Tags

NA

Expression

Mutation

Promoter

cfos minimal promoter

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTyr-TAG

Plasmid

#228782

Purpose

EcTyr aaRS/tRNA system for mammalian ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
BstR

Use

Baculoviral

Tags

Expression

Mammalian

Mutation

TAG suppressor

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_CRFmut

Plasmid

#208656

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-mSox2C17

Plasmid

#206369

Purpose

Expresses mouse SOX2-C17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox2-C17 chimera (Sox2, Sox17 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet1-LanCL2-C321A/C367A-C-Ter-His

Plasmid

#154188

Purpose

To express LanCL2-C321A/C367A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys321->Ala321; Cys367->Ala367

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-PHF21A__CAMK1D

Plasmid

#116831

Purpose

Lentiviral expression of PHF21A__CAMK1D fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PHF21A__CAMK1D

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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