We narrowed to 60,636 results for: Eng

1. AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

   Plasmid

   #65454

   Purpose

   Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Mutation

   H134R variant

   Promoter

   hSyn1

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBoBi-hLAMP2-C-PEN2

   Plasmid

   #183646

   Purpose

   Lentiviral expression of a lysosome-residential PEN2.

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 and LAMP2 (PSENEN, LAMP2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   WT

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBoBi-hTOMM20-C-PEN2

   Plasmid

   #183647

   Purpose

   Lentiviral expression of a mitochondrion-residential PEN2.

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 and TOMM20 (PSENEN, TOMM20 Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL1 sgRNA(MS2)-MS2-AIDmono

   Plasmid

   #112127

   Purpose

   empty sgRNA cloning vector with MS2-AIDmono

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, H130A/R131E…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL3 sgRNA(MS2)-MS2-AID3f

   Plasmid

   #112129

   Purpose

   empty sgRNA cloning vector with MS2-AID3f

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AIDmono 3f mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, F115Y/C116F…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL2 sgRNA(MS2)-MS2-AID3c

   Plasmid

   #112128

   Purpose

   empty sgRNA cloning vector with MS2-AID3c

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono 3c mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, F115Y/C116F…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pvalb-2A-Dre Flp-in replacement vector

   Plasmid

   #61571

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-TetO-hSOX2-C17

   Plasmid

   #206385

   Purpose

   Expresses human SOX2-C17 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

   Insert

   SOX2-C17 chimera (SOX2, SOX17 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.3-hAP1 1-419

   Plasmid

   #183644

   Purpose

   To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   ATP6AP1 (ATP6AP1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   truncated ATP6AP1 mutant; contains only amino aci…

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBoBi-hAP1 Δ420-440

    Plasmid

    #183642

    Purpose

    To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 (ATP6AP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    truncated ATP6AP1 mutant lacking the transmembran…

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBoBi-hSEC61B-C-PEN2

    Plasmid

    #183648

    Purpose

    Lentiviral expression of a ER-residential PEN2.

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 and SEC61B (PSENEN, SEC61B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    WT

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

    Plasmid

    #192552

    Purpose

    Expresses nuclear localized tdTomato under CAG promoter

    Depositor

    Guoping Feng

    Has Service

    AAV PHP.eB and AAV9

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    NLS

    Mutation

    deleted E273 and G274 compared to FPbase ID: PGG5S

    Promoter

    CAG

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNN_v2

    Plasmid

    #32182

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer NN
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHD_v2

    Plasmid

    #32183

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer HD
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNG_v2

    Plasmid

    #32181

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer NG
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNI_v2

    Plasmid

    #32180

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Monomer NI
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTALEN_v2 (NG)

    Plasmid

    #32190

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, NG for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTALEN_v2 (HD)

    Plasmid

    #32192

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, HD for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTALEN_v2 (NI)

    Plasmid

    #32189

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, NI for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTALETF_v2 (NN)

    Plasmid

    #32185

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Transcriptional Activator Backbone, NN for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge