We narrowed to 8,219 results for: Lif

Showing: 3081 - 3100 of 8219 results

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pJET-41-DExCon-antiGFPnanobody-mCherry-R11b

Plasmid

#179901

Purpose

Donor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

antiGFPnanobody-mCherry

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-AP-His

Plasmid

#71942

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Ankyrin [N388A/60R]

Plasmid

#128640

Purpose

Mammalian Expression Plasmid of anti-Pan-Ankyrin. Derived from hybridoma N388A/60.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Ankyrin Pan recombinant mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_R133C-HaloTag

Plasmid

#164064

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R133C mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 Isoform 1 R133C (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TOX2

Plasmid

#192920

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of TOX2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

TOX2 (TOX2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn iCyt_BrEt_SnFR_PM

Plasmid

#177757

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_PM

Use

AAV

Promoter

hSyn

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-GABARα1N-HA

Plasmid

#128110

Purpose

Express GABARα1 N terminal domain

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Gabra1 (Gabra1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Flrt2-AP-His

Plasmid

#71948

Purpose

Expresses the extracellular region of the FLRT2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt2 (Flrt2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-ZFPM2

Plasmid

#192922

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of ZFPM2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

ZFPM2 (ZFPM2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

Plasmid

#179428

Purpose

Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-(nospacer)-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-Fc-His

Plasmid

#72068

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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FKBP-DCRD-YFP

Plasmid

#87450

Purpose

rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

DCRD (distal C-terminal regulatory domain) (CACNA1F Human)

Tags

6xHis-T7, FKBP, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-Venus-FLARE-AKAR

Plasmid

#123331

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-Venus-FLARE-AKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

Plasmid

#123342

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-EKAR-EV

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-SVOP [N356/23R]

Plasmid

#114499

Purpose

Mammalian Expression Plasmid of anti-SVOP (Rat). Derived from hybridoma N356/23.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SVOP (Rattus norvegicus) recombinant mouse monoclonal antibody (Svop Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-SCG10/Stathmin-2 [L5/1R]

Plasmid

#140065

Purpose

Mammalian Expression Plasmid of anti-SCG10/Stathmin-2 (Rat). Derived from hybridoma L5/1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SCG10/Stathmin-2 (Rat) recombinant mouse monoclonal antibody (Stmn2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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Islr-AP-His

Plasmid

#71950

Purpose

Expresses the entire Islr protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Islr (Islr Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -0

Plasmid

#173889

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; gcry1:BFP -1

Plasmid

#173890

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; gcry1: BFP

Use

Synthetic Biology

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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C211

Plasmid

#174360

Purpose

For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (PABPC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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