We narrowed to 31,676 results for: puromycin

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Human sgRNA library Gattinara in pRDA_118 backbone

Pooled Library

#136986

Purpose

Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brunello human genome-wide library.

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only
pLenti-xFNLS-P2A-Puro

Plasmid

#110872

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-xFNLS-P2A-Puro

Plasmid

#110872

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS(3.7)

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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PresentER-Cassette mCherry

Plasmid

#102943

Purpose

PresentER with removable cassette for cloning of MHC-I antigen (mCherry version)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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PresentER-Cassette mCherry

Plasmid

#102943

Purpose

PresentER with removable cassette for cloning of MHC-I antigen (mCherry version)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mRuby3-IRES-puro

Plasmid

#85144

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)

Use

Lentiviral

Tags

mRuby3

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-CDH5-mRuby3-IRES-puro

Plasmid

#85144

Purpose

lentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3 (CDH5 Human)

Use

Lentiviral

Tags

mRuby3

Promoter

CAG

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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mCRISPRa-v2, genome-wide, top 5 sgRNAs/gene

Pooled Library

#83996

Purpose

Version 2 of the mouse CRISPRa genome wide library

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Use

Lentiviral

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only
pLV_Zika_NS2B_Flag

Plasmid

#79637

Purpose

Third generation Self-inactivating lentivector expressing NS2B from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS2B

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLV_Zika_NS2B_Flag

Plasmid

#79637

Purpose

Third generation Self-inactivating lentivector expressing NS2B from Zika virus

Depositor

Vaithi Arumugaswami

Insert

NS2B

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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LV-TRE-Empty-T2A-dsRedExpress2

Plasmid

#60623

Purpose

Expresses dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Empty

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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LV-TRE-Empty-T2A-dsRedExpress2

Plasmid

#60623

Purpose

Expresses dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Empty

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pDR-GFPuniv

Plasmid

#46085

Purpose

In vivo recombination assay fluorescent reporter

Depositor

Raymond Monnat

Article

Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

Insert

DR-GFPuniv reporter

Use

Recombination reporter plasmid

Expression

Mammalian

Mutation

E5G and K163R mutations in EGFP relative to wild-…

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAc-y1sgRNA-Cas9

Plasmid

#49331

Purpose

Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA
y1 sgRNA (y Fly)

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pAc-y1sgRNA-Cas9

Plasmid

#49331

Purpose

Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA
y1 sgRNA (y Fly)

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-Stx17TM

Plasmid

#45910

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

GFP

Mutation

deleted amino acids 1-228

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
pMRXIP GFP-Stx17TM

Plasmid

#45910

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

GFP

Mutation

deleted amino acids 1-228

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.1 shRUNX1 puro

Plasmid

#45816

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

Runx1 (RUNX1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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