We narrowed to 16,615 results for: puromycin

1. qTAG-KO-PGK-Puro

   Plasmid

   #227264

   Purpose

   Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBpuro-ERK-KTR-mCherry

   Plasmid

   #214829

   Purpose

   Encoding a fluorescent reporter for ERK activity

   Depositor

   Michiyuki Matsuda

   Article

   Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

   Insert

   ERK KTR-mCherry
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi 41BBL-CD86

   Plasmid

   #183244

   Purpose

   Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   CD86, 41BBL
   

   Expression

   Mammalian

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. SOX2se-CPG-TV

   Plasmid

   #70155

   Purpose

   a targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyA

   Depositor

   Rudolf Jaenisch

   Article

   Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

   Insert

   5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
   

   Use

   Mouse Targeting and Synthetic Biology

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF05J

   Plasmid

   #42941

   Depositor

   Eline Luning Prak

   Article

   Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

   Insert

   Negative control Functionally Dead Human L1 element (L1RE1 Human)
   

   Tags

   EGFP (opposite strand)

   Expression

   Mammalian

   Mutation

   ORF1 R261A, R262A

   Available Since

   April 25, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pLEX-FHH-Empty Vector-IRES-Puro

   Plasmid

   #120568

   Purpose

   For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Flag-HA-6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dazl-CPG-TV

   Plasmid

   #70144

   Purpose

   a targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyA

   Depositor

   Rudolf Jaenisch

   Article

   Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

   Insert

   5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
   

   Use

   Mouse Targeting and Synthetic Biology

   Available Since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1 LAMP1-mNeonGreen HRD

   Plasmid

   #207549

   Purpose

   Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible LAMP1-mNeonGreen
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LZRS-DNMT1

   Plasmid

   #24952

   Depositor

   Paul Khavari

   Article

   Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   Retroviral

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIP-AMPKa1_WT

    Plasmid

    #79010

    Purpose

    For mammalian expression of WT AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Expression

    Mammalian

    Promoter

    pCAGGs

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-puro-SNAP-FLAG-CD59

    Plasmid

    #50374

    Purpose

    Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Expression

    Mammalian

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211463

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-dead
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D211A, E225A, K227A

    Promoter

    TRE

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF806-reci

    Plasmid

    #186711

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-N-Puro-3xHA

    Plasmid

    #207696

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pBABE puro PPAR delta

    Plasmid

    #8891

    Depositor

    Bruce Spiegelman

    Article

    Brun et al Genes Dev. 1996 Apr 15. 10(8):974-84.

    Insert

    PPAR delta (Ppard Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRXIP GFP-SNAP29

    Plasmid

    #45923

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    synaptosomal-associated protein, 29kDa (SNAP29 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1 Flag Raptor

    Plasmid

    #26633

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    raptor (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

    Plasmid

    #228364

    Purpose

    mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Enascas12a nuclease and en…

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV JMJD3

    Plasmid

    #21212

    Depositor

    Paul Khavari

    Article

    Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. Halo-ATG16L1 HRD

    Plasmid

    #207537

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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