We narrowed to 6,239 results for: nls

1. RP1B FUS 1-163

   Plasmid

   #127192

   Purpose

   Protein expression for affinity purification

   Depositor

   Nicolas Fawzi

   Article

   Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

   Insert

   FUS 1-163 (FUS Human)
   

   Tags

   hexahistidine

   Expression

   Bacterial

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL1

   Plasmid

   #69496

   Purpose

   pJL1 expression vector with sfGFP

   Depositor

   Michael Jewett

   Article

   Martin et al Nat Commun. 2018 Mar 23;9(1):1203. doi:

   Insert

   Super folder GFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2

   Plasmid

   #218206

   Purpose

   Zebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   +15.2prox1a
   

   Use

   Zebrafish expression

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ1658-dCas9-EGFP

   Plasmid

   #51023

   Purpose

   Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

   Depositor

   Stanley Qi, Bo Huang

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   dCas9 fuse to EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   MSCV LTR promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDG1-PHP.eB

   Plasmid

   #220799

   Purpose

   AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   E4, E2A, VA, AAV2 rep and PHP.eB cap genes
   

   Use

   AAV; Aav helper vector

   Expression

   Mammalian

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-dCasRx-ALKBH5-PURO

   Plasmid

   #175582

   Purpose

   Expresses dCasRx-ALKBH5 in mammalian cell

   Depositor

   Qi Xie

   Article

   Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

   Insert

   dCasRx-ALKBH5
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   RfxCas13d R239A/H244A/R858A/H863A

   Promoter

   pMSCV

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

   Plasmid

   #84243

   Purpose

   Expresses ABA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sa dCas9
   PYL1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYTRW22K_7Ti1

   Plasmid

   #177293

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   lacZ
   

   Use

   Synthetic Biology; Yeast expression, rrn genomic …

   Expression

   Bacterial

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hygro-Cas9 donor

   Plasmid

   #86883

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection

   Depositor

   Danwei Huangfu

   Article

   Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-SpCas9-NG

    Plasmid

    #117919

    Purpose

    Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Expression

    Mammalian

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHEN-CoV2-nanobody-G6

    Plasmid

    #194589

    Purpose

    Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    G6 (S )
    

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Sniper2L

    Plasmid

    #193856

    Purpose

    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/

    Insert

    Sniper2L
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    E1007L

    Promoter

    EFS

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tet1-dCas9

    Plasmid

    #136650

    Purpose

    Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Tet1-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3TS nErCas12an

    Plasmid

    #132642

    Purpose

    Used for in vitro transcription of dual NLS ErCas12a mRNA

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    CRISPR; In vitro transcription

    Tags

    SV40 NLS

    Promoter

    T3

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid

    #107270

    Purpose

    Expresses Cas9 upon induction with doxicycline.

    Depositor

    Pablo Menéndez

    Article

    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

    Inserts

    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and TRE

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYTRW09K_0T5

    Plasmid

    #177281

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR

    Depositor

    Karl-Erich Jaeger

    Article

    Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

    Insert

    Ptet-tetA(C)
    

    Use

    Synthetic Biology; Yeast expression, tn5 genomic …

    Expression

    Bacterial

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVi U6_gRNA CMV_SadCas9-KRAB

    Plasmid

    #214609

    Purpose

    All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

    Depositor

    Ralf Gilsbach

    Article

    Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

    Inserts

    gRNA
    SadCas9
    

    Use

    AAV

    Tags

    NP NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CMV and U6

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid

    #101178

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/H698A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSaCas9-1xms2-2x3’UTR

    Plasmid

    #122946

    Purpose

    For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

    Depositor

    Baisong Lu

    Article

    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

    Insert

    SaCas9-MS2-HBB 3' UTR
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSaCas9-1xPP7-2x3'UTR

    Plasmid

    #122947

    Purpose

    For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR

    Depositor

    Baisong Lu

    Article

    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

    Insert

    SaCas9-PP7-HBB 3' UTR
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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