Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 18,824 results for: nls

Clear keywords
Showing: 3101 - 3120 of 18824 results

  1. pFC482

    Plasmid
    #246439
    Purpose
    The plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signal
    Depositor
    Ron Weiss
    Publication
    Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038
    Insert
    CasE
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SapI domesticated
    Promoter
    EF-1a
    Available since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246439-plasmid-map-sequence-id-492325

    Enlarge

  3. APEX2 VLP

    Plasmid
    #182211
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-APEX2
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology; Tem imaging
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182211-plasmid-map-sequence-id-357716

    Enlarge

  6. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid
    #176458
    Purpose
    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter
    Depositor
    Hui Yang
    Publication
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176458-plasmid-map-sequence-id-346303

    Enlarge

  8. p4B5-EGFP

    Plasmid
    #140324
    Purpose
    Detection of Dengue virus infection in live cells
    Depositor
    Alan Rothman
    Publication
    Medin et al J Virol Methods. 2015 Jan;211:55-62. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    2 conservative n.t. differences near 5' end …
    Promoter
    CMV
    Available since
    May 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    140324-plasmid-map-sequence-id-296784

    Enlarge

  9. pCE059-SiT-ddCas12a(E993A)-1xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202042
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a NLP nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Publication
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-1xNLS-[Activ]
    Expression
    Mammalian
    Available since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202042-plasmid-map-sequence-id-401647

    Enlarge

  10. pCE059-SiT-ddCas12a(E993A)-3xNLS-[Activ]-triplex-DR-M13-DR-pA

    Plasmid
    #202043
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and [Activ] transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Publication
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-3xNLS-[Activ]
    Expression
    Mammalian
    Available since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202043-plasmid-map-sequence-id-401604

    Enlarge

  11. pCE059-SiT-ddCas12a(E993A)-3xNLS-VP64-triplex-DR-M13-DR-pA

    Plasmid
    #202044
    Purpose
    Encodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.
    Depositor
    Darren Cusanovich
    Publication
    Kang et al ACS Synth Biol. 2025 May 16;14(5):1732-1
    Insert
    ddCas12a(E993A)-3xNLS-VP64
    Expression
    Mammalian
    Available since
    Aug. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    202044-plasmid-map-sequence-id-401598

    Enlarge

  12. pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest

    Plasmid
    #240111
    Purpose
    A lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
    Depositor
    Achim Kramer
    Publication
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+Electra2-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added JeT Promoter
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240111-plasmid-map-sequence-id-481759

    Enlarge

Showing: 3101 - 3120 of 18824 results