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BII-Sh-PnGW-CMV

Plasmid

#133366

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-CMV-Luc

Plasmid

#133367

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PIR702W-Luc

Plasmid

#133362

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IRFP-702

Tags

HA-tagged IRFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PIR702W

Plasmid

#133361

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

IRFP-702

Tags

HA-tagged IRFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BII-Sh-PnGW-Luc

Plasmid

#133360

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_92_positive_Control

Plasmid

#130573

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC4

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Cerulean_Nanotag_91_positive_Control

Plasmid

#130572

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC3

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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JK104: pMVP-DEST

Plasmid

#121851

Purpose

pMVP destination vector, empty expression plasmid backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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gh144

Plasmid

#106803

Purpose

expression of gRNA targeting LARGE

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

LARGE

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh119

Plasmid

#106789

Purpose

expression of gRNA targeting GCNT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GCNT6

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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gh20

Plasmid

#106704

Purpose

expression of gRNA targeting GALNT20

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT20

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCF736

Plasmid

#121893

Purpose

EF1a-dTEV-protease-GFP

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

TEV protease
eGFP

Use

Lentiviral

Promoter

EF-1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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gh163

Plasmid

#106816

Purpose

expression of gRNA targeting TMTC4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

TMTC4 (TMTC4 Human)

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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gh41

Plasmid

#106715

Purpose

expression of gRNA targeting ST6GAL2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GAL2 (ST6GAL2 Human)

Expression

Mammalian

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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gh156

Plasmid

#106823

Purpose

expression of gRNA targeting GYLT1B (LARGE2)

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GYLT1B (LARGE2)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR I206F (JBEI-3678)

Plasmid

#110149

Purpose

SaGGR site-directed mutation of I206F

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of I206F

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR G91H (JBEI-3677)

Plasmid

#110148

Purpose

SaGGR site-directed mutation of G91H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of G91H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR L377H (JBEI-3683)

Plasmid

#110150

Purpose

SaGGR site-directed mutation of L377H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of L377H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-mSPDEF deltaNC2

Plasmid

#100869

Purpose

Mammalian expression of mouse SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Spdef (Spdef Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

truncated protein 70-238aa

Promoter

CMV

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-mSPDEF deltaC2

Plasmid

#100867

Purpose

Mammalian expression of mouse SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Spdef (Spdef Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

truncated protein 1-238aa

Promoter

CMV

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge