Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,549 results for: erf

Clear keywords
Showing: 3121 - 3140 of 4549 results

  1. C92m

    Plasmid
    #121000
    Purpose
    MoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).
    Depositor
    Richard Murray
    Insert
    sfCFPpdt3 (Chen,Bennett)
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121000-plasmid-map-sequence-id-238058

    Enlarge

  2. C101m

    Plasmid
    #121005
    Purpose
    MoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).
    Depositor
    Richard Murray
    Insert
    sfCFP with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121005-plasmid-map-sequence-id-236158

    Enlarge

  3. C20m

    Plasmid
    #120961
    Purpose
    MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)
    Depositor
    Richard Murray
    Insert
    sfYFP-ssrA (has BbsI site)
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120961-plasmid-map-sequence-id-235979

    Enlarge

  5. PB CAG-F-bpA-3xSV40pA-F-td-sfGFP

    Plasmid
    #133573
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133573-plasmid-map-sequence-id-274063

    Enlarge

  6. pExp-TF

    Plasmid
    #112575
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-TriggerFactor and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112575-plasmid-map-sequence-id-217211

    Enlarge

  7. PB CAG-L-bpA-3xSV40pA-L-td-sfGFP

    Plasmid
    #133577
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133577-plasmid-map-sequence-id-274079

    Enlarge

  8. PB CAG-F-bpA-2xSV40pA-F-td-sfGFP

    Plasmid
    #133572
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133572-plasmid-map-sequence-id-274057

    Enlarge

  9. PB CAG-L-bpA-2xSV40pA-L-td-sfGFP

    Plasmid
    #133576
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133576-plasmid-map-sequence-id-274071

    Enlarge

  10. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #133575
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133575-plasmid-map-sequence-id-274068

    Enlarge

  11. pUPD2 dCas9 (GB2664)

    Plasmid
    #160596
    Purpose
    CDS of dCas9 with B3 nomenclature
    Depositor
    Diego Orzaez
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160596-plasmid-map-sequence-id-322070

    Enlarge

  12. PB CAG-R-bpA-2xSV40pA-R-td-sfGFP

    Plasmid
    #133578
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (bpA 2x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre/rox
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133578-plasmid-map-sequence-id-274080

    Enlarge

  13. PB CAG-F-bpA-SV40pA-F-td-sfGFP

    Plasmid
    #133571
    Purpose
    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Flp/frt
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133571-plasmid-map-sequence-id-274056

    Enlarge

  14. C100m

    Plasmid
    #121004
    Purpose
    MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121004-plasmid-map-sequence-id-236143

    Enlarge

  15. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

    Enlarge

  16. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid
    #217304
    Purpose
    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter
    Depositor
    Luke Gilbert
    Insert
    dCas9-tagBFP-KRAB
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Promoter
    Ef1alpha
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217304-plasmid-map-sequence-id-429717

    Enlarge

  17. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid
    #192663
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192663-plasmid-map-sequence-id-379915

    Enlarge

  18. pExp-sfGFP

    Plasmid
    #112573
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and sfGFP
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112573-plasmid-map-sequence-id-217227

    Enlarge

  19. pTH385-GST-TEV-SUP35

    Plasmid
    #29744
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    wild type
    Available Since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    29744-plasmid-map-sequence-id-13828

    Enlarge

  20. pTH278-GST-TEV-SUP45

    Plasmid
    #29743
    Depositor
    Tobias von der Haar
    Insert
    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    wild type
    Available Since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    29743-plasmid-map-sequence-id-13827

    Enlarge

Showing: 3121 - 3140 of 4549 results