We narrowed to 6,903 results for: plasmid dna

1. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

   Plasmid

   #167295

   Purpose

   Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   BAK (BAK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4398

   Plasmid

   #74042

   Purpose

   Expresses human codon-optimized LbCpf1 and Lb crRNA.

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Lb crRNA
   LbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFETCh_AHR

   Plasmid

   #101071

   Purpose

   Donor vector for 3' FLAG tag of human AHR

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   AHR homology arms (AHR Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-gRNA

   Plasmid

   #158973

   Purpose

   A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

   Depositor

   Charles P. Lai

   Article

   Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

   Insert

   gRNA3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiV2-EF1α-nCas9/RT

   Plasmid

   #210188

   Purpose

   Expression of nCas9/RT-P2A-Puro

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   nCas9/RT-P2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-Sa-SauriCas9

   Plasmid

   #135967

   Purpose

   Expresses Sa-SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL410_Ins2500

   Plasmid

   #49058

   Purpose

   Expresses luciferase under control of mouse insulin 2 gene promoter

   Depositor

   Kevin Ferreri

   Article

   Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

   Insert

   mouse Ins2 promoter (Ins2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ins2 promoter

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMKO.1 puro GFP shRNA

   Plasmid

   #10675

   Purpose

   Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   GFP shRNA
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. p15_attB(bxb)_lox

   Plasmid

   #183764

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE4396

    Plasmid

    #74041

    Purpose

    Expresses human codon-optimized AsCpf1 and As crRNA.

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    As crRNA
    AsCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBR_attB(C31)_FRT

    Plasmid

    #183761

    Purpose

    phiC31-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-BAF_L58R

    Plasmid

    #101776

    Purpose

    Expresses EGFP tagged mutant BAF (L58R) in human cells

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    BAF (BANF1) (BANF1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L58R mutation

    Promoter

    EF1a

    Available Since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

    Plasmid

    #39454

    Depositor

    Hongjun Song

    Article

    Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

    Insert

    HA-hTet1CD (TET1 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1418–2136

    Promoter

    EF1a

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

14. SuExp His-Myc-mPLD3

    Plasmid

    #173853

    Purpose

    Protein expression plasmid for recombinant His-Myc tag mouse PLD3

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD3 (Pld3 Mouse)
    

    Use

    Tags

    6xHis, Myc, Factor X cleavable

    Expression

    Mammalian

    Mutation

    intracellular and transmembrane domain truncated,…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-BE4-PpAPOBEC1 R33A

    Plasmid

    #138345

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1 R33A

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1 R33A
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Change of Arg 33 to Ala.

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_AHR_1

    Plasmid

    #101076

    Purpose

    Encodes gRNA for 3' target of human AHR

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against AHR (AHR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 145-pGL3ctrl-3' UTR

    Plasmid

    #28175

    Depositor

    Michael Kastan

    Article

    Chen et al Genes Dev. 2010 Oct 1. 24(19):2146-56.

    Insert

    Luciferase flanked by tp53 UTRs (TP53 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Note that insert length is based on sum of the 2 …

    Promoter

    Available Since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA

    Plasmid

    #39455

    Depositor

    Hongjun Song

    Article

    Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

    Insert

    HA-hTet1CDmu (TET1 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1418–2136 with mutations H1672 to Y, 1674D to A

    Promoter

    EF1a

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pKD077

    Plasmid

    #136473

    Purpose

    SSB-mTur2 IDL fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-mTur2 (ssb E. coli)
    

    Use

    Tags

    mTurquoise2

    Expression

    Bacterial

    Mutation

    mTurquoise2 inserted between Phe148 and Ser149

    Promoter

    T7 promoter

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PVT1-luciferase

    Plasmid

    #113350

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    PVT1-enhancer (PVT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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