We narrowed to 9,935 results for: ada

1. pcDNA-ALFA-tag-Gal4-VP16

   Plasmid

   #199465

   Purpose

   Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

   Insert

   Gal4-ALFA-tag-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. NavBp

   Plasmid

   #100014

   Purpose

   Electrophysiology in mammalian cells: Mammalian expression of a voltage gated ion channel and GFP

   Depositor

   Paul DeCaen, David Clapham

   Article

   DeCaen et al Elife. 2014 Nov 11;3. doi: 10.7554/eLife

   Insert

   NavBp
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-SUN1_888

   Plasmid

   #125851

   Purpose

   Expresses SUN1_888 tagged with GFP in mammalian cells

   Depositor

   Miki Hieda

   Article

   Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

   Insert

   SUN1_888 (SUN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK-flag-USP19 C506S

   Plasmid

   #78584

   Purpose

   Expression flag-USP19 C506S in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 C506S (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   mutate 506 cysteine residue to serine

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-CAG-mNeptune2.5-P2A-GFP

   Plasmid

   #197156

   Purpose

   Expresses the protein of mNeptune2.5-P2A-GFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   mNeptune2.5-P2A-GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMJ823

   Plasmid

   #39313

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9 (lin2744 Listeria innocua)
   

   Use

   CRISPR

   Tags

   MBP and thioredoxin-His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCDH-EF1a-mTagBFP-T2A-TetOn3G

   Plasmid

   #179888

   Purpose

   Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

   Depositor

   Patrick Caswell

   Article

   Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

   Insert

   TetOn3G
   

   Use

   Lentiviral

   Tags

   mTagBFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-IREShygro2

   Plasmid

   #153966

   Purpose

   Expresses EGFP in mammalian cells

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   EGFP
   

   Use

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CMV promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sABE v3.22 Lenti_2

    Plasmid

    #209556

    Purpose

    pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pU6, pCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 S6K2 KR

    Plasmid

    #17730

    Depositor

    John Blenis

    Article

    Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

    Insert

    S6K2 (RPS6KB2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K99R: kinase dead mutant

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pRS415_pGal-nCas9(H840A)

    Plasmid

    #79616

    Purpose

    Expresses nCas9(H840A) in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H840A for nickase Cas9

    Promoter

    pGal1

    Available since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3XFlag-CMV10-Flag-mMcl-1

    Plasmid

    #32978

    Depositor

    Joseph Opferman

    Article

    Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

    Insert

    Mcl-1 (Mcl1 Mouse)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCfuNca-Plastid

    Plasmid

    #190818

    Purpose

    Plastid targeting GFP with ble resistant gene for zeocin

    Depositor

    Peter Kroth

    Article

    Sprecher et al Algal Research. 2023 May; Vol 72: 103136

    Inserts

    Diatom plastid targeted GFP
    Bleomycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Cylindrotheca fusiformis fcp and Nitzschia captiv…

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. px458_2A_GFP_sgRNA_ZNF598

    Plasmid

    #127121

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA ZNF598 (ZNF598 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-cT3G-cERP2-MEIOC

    Plasmid

    #222562

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    MEIOC (C17orf104 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEBG-TRAF2 (GST)

    Plasmid

    #21586

    Depositor

    John Kyriakis

    Article

    Chadee et al Mol Cell Biol. 2002 Feb . 22(3):737-49.

    Insert

    TNF receptor-associated factor 2 (TRAF2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pLJM1 Flag Raptor HRas25

    Plasmid

    #26637

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    raptor-HRas25 (RPTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pJW1354

    Plasmid

    #69490

    Purpose

    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms

    Depositor

    Jordan Ward

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    

    Use

    CRISPR; Template to generate cassettes for -media…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. LRP6 deltaN-pCS2-VSVG

    Plasmid

    #27281

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Expression

    Mutation

    contains amino acid residues 1370–1613 (extracell…

    Promoter

    Available since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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