We narrowed to 10,595 results for: ada

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-TK-Firefly-Control

Plasmid

#122277

Purpose

Lentiviral vector for stable expression of Firefly luciferase.

Depositor

Demián Cazalla

Article

Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

Insert

Firefly luciferase

Use

Lentiviral and Luciferase

Expression

Bacterial

Promoter

Thymidine kinase

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Tubulin.K40Q

Plasmid

#32912

Depositor

Tso-Pang Yao

Article

Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

Insert

Tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40Q

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-SUN1_785

Plasmid

#125852

Purpose

Expresses SUN1_785 tagged with GFP in mammalian cells

Depositor

Miki Hieda

Article

Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

Insert

SUN1_785 (SUN1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-Nedd4/2795

Plasmid

#11426

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

D661N compared to GenBank reference sequence U966…

Promoter

CMV

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pSTAR-mOrange2-sfGFP

Plasmid

#115693

Purpose

Generation of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent Timer

Depositor

David Suter

Article

Alber et al Mol Cell. 2018 Aug 17. pii: S1097-2765(1

Insert

SA-mOrange2-sfGFP-SD

Use

Lentiviral

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_N

Plasmid

#209553

Purpose

pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

TadA-8e(2-76)-2xFRB

Expression

Mammalian

Promoter

pCMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_C

Plasmid

#209554

Purpose

pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

2xFKBP-TadA-8e(77-167)-SpCas9(D10A)

Expression

Mammalian

Promoter

pCMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DDX4

Plasmid

#222527

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DDX4 (DDX4 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBa.Lamp1-GFP

Plasmid

#180250

Purpose

Mammalian expression of LAMP1

Depositor

Marvin Bentley

Article

Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

Insert

LAMP1 (LAMP1 Human)

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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EK0396 BFPmut-stop-t70587 (pEAQ)

Plasmid

#191171

Purpose

EK0208 in plant-expressable format; Trigger 1 in 3' UTR of an inactive BFP

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

t70587 (plant)

Expression

Plant

Mutation

WT

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-EFS-Blast

Plasmid

#227260

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP718nano

Plasmid

#195744

Purpose

Monomeric near-infrared fluorescent protein miRFP718nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-mRuby3

Plasmid

#171154

Purpose

Encodes mRuby3 fluorophore

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

mRuby3

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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xABEmax

Plasmid

#119813

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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V51 pIRESpuro-GLUE (pGLUE)

Plasmid

#15100

Purpose

tandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tags

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-EFS-Zeo

Plasmid

#227258

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-AdEvolve-DEST

Plasmid

#131692

Purpose

A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Type

Empty backbone

Use

Adenoviral

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-NHX1-HaloTagx2

Plasmid

#177107

Purpose

Expresses Nhx1-HaloTagx2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

NHX1 (NHX1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS10

Plasmid

#127129

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS10 (RPS10 Human)

Use

Lentiviral

Mutation

K24R- please see depositor comments

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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