We narrowed to 5,405 results for: dre

1. JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

   Plasmid

   #156406

   Purpose

   Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12V (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   mScarlet-I

   Expression

   Mutation

   mutant KRAS4A-G12V

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

   Plasmid

   #162878

   Purpose

   Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

   Plasmid

   #156414

   Purpose

   kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   EGFP

   Expression

   Mutation

   mutant KRAS4B-G12V

   Promoter

   kdrl

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_BRD003-sgPOLR2D

   Plasmid

   #125769

   Purpose

   constitutive expression of a guide RNA targeting human POLR2D (CRISPR positive control)

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   sgPOLR2D (POLR2D Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL

   Plasmid

   #73210

   Purpose

   Expresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

   Insert

   KozATG-myc-dL5-2XG4S-mCer3-SKL (MYC Synthetic, Human)
   

   Use

   Tags

   The FAP and mCerulean3 are fused with 2 copies of…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMVtrunc msfGFPΔC10-Utr28-222

   Plasmid

   #231558

   Purpose

   Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Utrophin calponin homology domain (UTRN Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i3-14-GFP10

   Plasmid

   #180349

   Purpose

   mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRIP TRE BI_SEPT9_i3-14-GFP11_SEPT9_i3-14-GFP10

   Plasmid

   #180352

   Purpose

   mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT9_i3 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN9_v3 (SEPTIN9 Human)
   SEPTIN9_v3 (SEPTIN9 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP TRE BI_GFP10-14-SEPT7 Gmut2_GFP11-14-SEPT7 Gmut2

   Plasmid

   #180353

   Purpose

   mammalian co-expression of human SEPT7 Gmut2 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN7 (SEPTIN7 Human)
   IRES-TagBFP
   

   Use

   Lentiviral

   Tags

   GFP10 and GFP11

   Expression

   Mammalian

   Mutation

   SEPT7 H279D

   Promoter

   bidirectional TRE

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIP TRE BI_SEPT9_i1-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid

    #180359

    Purpose

    mammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT7 H279D

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRIP TRE BI_SEPT9_i3-14-GFP10_GFP11-14-SEPT7 Gmut2

    Plasmid

    #180361

    Purpose

    mammalian co-expression of human SEPT9_i3 fused to GFP10 and of human SEPT7 Gmut2 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT7 H279D

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIP TRE BI_GFP11-14-SEPT7_GFP10-14-SEPT7

    Plasmid

    #180347

    Purpose

    mammalian co-expression of human SEPT7 fused to GFP10 and of human SEPT7 fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN7 (SEPTIN7 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP_SEPT6

    Plasmid

    #180312

    Purpose

    bacterial co-expression of human SEPT2 NCmut fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    SEPT2 F20D, V27D

    Promoter

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid

    #176141

    Purpose

    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    XRCC1-T2A-PolB (XRCC1, POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP and MYC

    Expression

    Mammalian

    Mutation

    Myc-tagged PolB with mutation in Asp256 to Ala, a…

    Promoter

    EF1A

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

    Plasmid

    #176140

    Purpose

    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    XRCC1-T2A-PolB (XRCC1, POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP and MYC

    Expression

    Mammalian

    Mutation

    Myc-tagged PolB with mutations Lys35 to Ala, Lys6…

    Promoter

    EF1A

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

    Plasmid

    #156413

    Purpose

    kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    kdrl

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

    Plasmid

    #156404

    Purpose

    Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mTagBFP2

    Expression

    Mutation

    mutant KRAS4A-G12D

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-MerCreMer-hygro

    Plasmid

    #188982

    Purpose

    Retroviral expression of tamoxifen-inducible Cre recombinase

    Depositor

    William Kaelin

    Article

    Shi et al Cancer Cell. 2022 Sep 12;40(9):939-956.e

    Insert

    Tamoxifen-inducible Cre recombinase
    

    Use

    Cre/Lox and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS1384

    Plasmid

    #29304

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).

    Depositor

    Elizabeth Simpson

    Article

    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

    Insert

    Ple185
    

    Use

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

    Plasmid

    #180357

    Purpose

    mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN 2 (SEPTIN2 Human)
    IRES-TagBFP
    

    Use

    Lentiviral

    Tags

    GFP10 and GFP11

    Expression

    Mammalian

    Mutation

    SEPT2 F20D, V27D

    Promoter

    bidirectional TRE

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge