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We narrowed to 3,230 results for: eed

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Showing: 3141 - 3160 of 3230 results

  2. pFGC-I2Cas9

    Plasmid
    #173158
    Purpose
    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.
    Depositor
    Piotr Ziolkowski
    Article
    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:
    Inserts
    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    Tags
    3xFLAG-NLS and NLS
    Expression
    Plant
    Mutation
    Contains a D169N mutation with no visible effect …
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    173158-plasmid-map-sequence-id-358594

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  4. Desmoplakin I acceptor only control (F40-based tension sensor)

    Plasmid
    #119189
    Purpose
    The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119189-plasmid-map-sequence-id-230531

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  7. Myc-gamma2S/deltaIL

    Plasmid
    #119730
    Purpose
    GABAA receptor expression (chimeric rat gamma2 short subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat delta subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Article
    Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.
    Insert
    GABRG2/GABRD (Gabrg2, Gabrd Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    119730-plasmid-map-sequence-id-229624

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  8. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid
    #119188
    Purpose
    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119188-plasmid-map-sequence-id-230532

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  10. Myc-gamma2/deltaILTM4

    Plasmid
    #119735
    Purpose
    GABAA receptor expression (chimeric rat rat gamma 2 subunit whose large intracellular loop, TM4 and C-term have been replaced with the equivalent domains of the rat delta subunit) C-myc near N-term
    Depositor
    Angel de Blas
    Article
    Wu et al J Biol Chem. 2012 Aug 10;287(33):27417-3
    Insert
    GABRG2/GABRD (Gabrg2, Gabrd Rat)
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    119735-plasmid-map-sequence-id-229652

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  11. pUt-mNF-BACH1

    Plasmid
    #199219
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV D2ir promoter
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    199219-plasmid-map-sequence-id-396467

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  13. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid
    #199221
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    tight TRE promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199221-plasmid-map-sequence-id-396541

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  14. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid
    #199218
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    CMV D2ir promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199218-plasmid-map-sequence-id-396470

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Showing: 3141 - 3160 of 3230 results