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pSBbi-moxGFP-P2A-T2A-cMyc-Puro

Plasmid

#176892

Purpose

Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

Depositor

Jorge Luis Arias-Arias

Article

Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

Insert

moxGFP-P2A-T2A-c-Myc

Use

Transposon

Tags

Myc and moxGFP

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

Plasmid

#75118

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the bromodomain of BRD7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

Plasmid

#204547

Purpose

Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

deltaUBA

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-V12-puro

Plasmid

#156168

Purpose

Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

Plasmid

#110830

Purpose

Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

AZGP1 (Azgp1 Mouse)

Use

Non-viral gene expression vector

Tags

T2A and dTomato

Promoter

CMV

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

Plasmid

#117411

Purpose

AsCas12a-gRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

AsCas12a gRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

Plasmid

#22023

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450A.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pBabe puro-HA-PIK3CA(H1047R;F486S;del)

Plasmid

#33255

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and phenylalan…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

Plasmid

#138525

Purpose

Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

Depositor

Richard Jenner

Article

Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

Insert

Control non-PRC2 binding A-tract repeat RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

Plasmid

#25948

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

Plasmid

#75123

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

3x FLAG-tag

Mutation

bromodomain replaced with the first bromodomain o…

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
pZIP-hCMV-ZsGreen-P2A-Puro-NT4

Plasmid

#123328

Purpose

Lentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

non-targeting control shRNA embedded in miR30 backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

Plasmid

#22018

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutation T17N.

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pLVX-Flag-Polß(D256A)-IRES-puro

Plasmid

#128677

Purpose

mammalian expression of Flag-Polß(D256A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D256A

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode1

Plasmid

#83678

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode1

Use

Retroviral

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) VOPP1

Plasmid

#107507

Purpose

expressing VOPP1, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

VOPP1 (VOPP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

Plasmid

#72258

Purpose

Express FLPe, copGFP and Puromycin resistance gene under CB promoter

Depositor

Kazuhiro Oka

Insert

FLPe-P2A-copGFp-T2A-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

CB and chicken beta-actin + CMV enhancer

Available Since

Jan. 15, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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