We narrowed to 166,561 results for: Gene

1. p101_CRISPRai_PiggyBac

   Plasmid

   #213777

   Purpose

   Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   VPR-dSaCas9
   dSpCas9-KRAB-BFP
   zeocin
   Tet-On transactivator
   

   Use

   CRISPR

   Promoter

   EF1a (EF-1-alpha intron a) and Tet_On_TRE

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPbU6_hdhfr/yfcu_Cas9

   Plasmid

   #216423

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Expression

   Bacterial

   Available Since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

   Plasmid

   #32702

   Purpose

   Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   eGFP
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMaCTag-P11

   Plasmid

   #120022

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-i

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mScarlet-i
   

   Use

   Pcr template

   Tags

   mScarlet-i

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-multi-CRISPR

   Plasmid

   #85402

   Purpose

   Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

   Depositor

   Qin Yan

   Article

   Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Vmn2r1-5Hprt-loxP TV

   Plasmid

   #108079

   Purpose

   Tergeting vector for Vmn2r1 gene knockout mice (∆C1 strain)

   Depositor

   Peter Mombaerts

   Article

   Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

   Insert

   Vmn2r1-Hprt (5')-loxP-neo (Vmn2r1 Mouse)
   

   Use

   Mouse Targeting

   Available Since

   May 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKL2299

   Plasmid

   #186332

   Purpose

   This plasmid carries the majority suite of Agrobacterium virulence genes from Ti plasmid pTiBo542. The additional copies of vir genes enhance Agrobacterium-mediated plant transformation.

   Depositor

   Kan Wang

   Article

   Kang et al Front Plant Sci. 2022 May 4;13:860971. d

   Insert

   virulence genes from Agrobacterium tumefaciens pTiBo542
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHeEf1a-mEGFP-NLS

   Plasmid

   #205012

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the ef1a gene from Hypsibius exemplaris
   

   Use

   Tardigrade expression

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSB161 - pL2_pSB90_2x35S::GUS::tMAS

   Plasmid

   #123197

   Purpose

   binary plant vector for transient expression of GUS (with introns)

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::GUS::tMAS
   

   Expression

   Plant

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. JD136

    Plasmid

    #229497

    Purpose

    Adenoviral helper plasmid containing E2A and L4-22k/33k and VA RNA I genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, L4-22k/33k, VA RNA I
    

    Use

    AAV and Adenoviral

    Expression

    Mammalian

    Promoter

    wt promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-P19

    Plasmid

    #124789

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry-mNeonGreen
    

    Use

    Pcr template

    Tags

    mCherry-mNeonGreen

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-idCas9-vpr

    Plasmid

    #89985

    Purpose

    inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

    Depositor

    Jie Na

    Article

    Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

    Insert

    dCas9-VPR
    

    Expression

    Mammalian

    Promoter

    TRE-tight

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mouse Clock delta 19

    Plasmid

    #177312

    Purpose

    expression of mutant mouse clock

    Depositor

    Joseph Takahashi

    Article

    King et al Cell. 1997 May 16;89(4):641-53. doi: 10.

    Insert

    mouse clock delta 19
    

    Expression

    Mammalian

    Mutation

    deletion from original mClock gene (514-564aa)

    Promoter

    CMV

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDOC-GG

    Plasmid

    #149377

    Purpose

    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Inserts

    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX_TRE3G_DD::mCherry

    Plasmid

    #108679

    Purpose

    Tunable system for controlled gene expression and protein stability in mammalian cells

    Depositor

    Lucia Marucci

    Article

    Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

    Insert

    Destabilizing Domain (DD) mCherry fusion protein
    

    Use

    Lentiviral

    Tags

    DD::mCherry fusion protein

    Expression

    Mammalian

    Promoter

    Doxycycline inducible

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

    Plasmid

    #216421

    Purpose

    Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    3xmyc

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPbU6_hdhfr/yfcu

    Plasmid

    #216422

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Expression

    Bacterial

    Available Since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC_mNG2(11)_BSDminus_F2

    Plasmid

    #184164

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAluYa5-neo-TET

    Plasmid

    #51283

    Purpose

    expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    AluYa5
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    upstream pol III enhancer sequence of the 7SL gen…

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

    Plasmid

    #89693

    Purpose

    Luciferase reporter plasmid targeted at csr-1 locus

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gsy-1 promoter, luciferase
    

    Use

    Yeast episomal vector

    Tags

    luciferase

    Promoter

    gsy-1

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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