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pHAGE2 mCherry-Rtn4a

Plasmid

#86683

Purpose

Lentivirus to stably express fluorescent human protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

Rtn4a (RTN4 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pEf1a-superDn29-P2A-GFP

Plasmid

#247152

Purpose

Mammalian expression of a human codon optimized engineered superDn29 recombinase (highest efficiency variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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KillerRed-LacI-NLS

Plasmid

#215102

Purpose

Expresses KillerRed-LacI-NLS in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

KillerRed-LacI-NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-hCD4

Plasmid

#162746

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD4 gene

Depositor

Maki Nakayama

Article

Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR P2R-P3-EGFP

Plasmid

#48349

Purpose

Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EGFP

Use

Gateway entry vector

Mutation

Aminoacid 40 is valine in our sequence, while it …

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pIVT-R2Tg-RTmut

Plasmid

#238106

Purpose

For IVT of RT-mutant R2Tg editor, codon optimized for mammalian expression.

Depositor

Feng Zhang

Article

Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

Insert

Reverse transcriptase mutant R2Tg, mammalian codon optimized

Use

For ivt

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

Plasmid

#157974

Purpose

Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

Depositor

Carolyn McBride

Article

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

Orco

Expression

Insect

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR-attL5-CRY2-mCh-attL2

Plasmid

#160438

Purpose

Entry vector for cloning Cryptochrome2-mCherry using 2-fragment gateway recombination

Depositor

Nicholas Tolwinski

Article

Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Bacterial

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-CA-mDia1

Plasmid

#45583

Depositor

Klaus Hahn, Ronen Zaidel-Bar

Article

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Insert

Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Insert mDia1 contains mutations V161D and N165D i…

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-FKBP-IRF3

Plasmid

#221260

Purpose

Expression of IRF3 in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRF3 (Irf3 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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p6600 MSCV-IP N-HAonly JUN

Plasmid

#34898

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

JUN (JUN Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
pRP.ExTri-CMV-5'UTR-ApoB48-Linker-eGFP

Plasmid

#138334

Purpose

Expresses APOB-Linker-eGFP

Depositor

Mahmood Hussain

Article

Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119

Insert

5'UTR-ApoB48-Linker-eGFP (APOB Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

Plasmid

#86230

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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p6608 MSCV-IP N-HAonly CHAF1B

Plasmid

#34901

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

CHAF1B (CHAF1B Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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L2096-GPA-BRET-Biosensor-Retrovirus-TD146

Plasmid

#222878

Purpose

Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO2-Flp

Plasmid

#149296

Purpose

Cre-dependent expression of Flp recombinase.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

Flp recombinase (flp Budding Yeast)

Use

AAV

Mutation

Derived from the mouse codon-optomized FlpO. The…

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-CIAO-Flp

Plasmid

#149294

Purpose

Cre-dependent expression of Flp recombinase.

Depositor

Edward Callaway

Article

Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

Insert

Flp recombinase (flp Budding Yeast)

Use

AAV

Mutation

Derived from the mouse codon-optomized FlpO. The…

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS

Plasmid

#221313

Purpose

Expression of MAVS in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBabepuro3-p16Flag

Plasmid

#24934

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p16 (CDKN2A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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pB PUb-integrase

Plasmid

#183966

Purpose

piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

PhiC31 integrase

Use

Piggybac insect transgenesis destination vector

Promoter

Aedes aegypti Polyubiquitin

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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