We narrowed to 5,312 results for: pcDNA3.1(+)

1. TNXXL-CTS20

   Plasmid

   #48342

   Purpose

   FRET reporter for Ca2+ inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   CTS20
   

   Use

   Tags

   TN-XXL

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

2. tCRMod-GGSGGS7

   Plasmid

   #111761

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS7-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. tCRMod-GGSGGS5

   Plasmid

   #111760

   Purpose

   Expresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domain

   Depositor

   Brenton Hoffman

   Article

   LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

   Insert

   Clover-GGSGGS5-mRuby2 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOVC1_X_ScPH1-S

   Plasmid

   #131193

   Purpose

   Expresses ScPH1-S (Synechosystis PCC6803) preceded by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   ScPH1-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. [Bla][IRES][GFP]

   Plasmid

   #97193

   Purpose

   Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

   Depositor

   Edward Boyden

   Article

   Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Inserts

   beta-Lactamase
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3-MARS-p85(iSH2)

   Plasmid

   #205236

   Purpose

   Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

   Depositor

   Jeremy Baskin

   Inserts

   PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
   p85(iSH2) (Pik3r1 Mouse)
   

   Use

   Tags

   Nuclear Export Sequence and mCherry

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Integrin-αv Ectodomain M400C

   Plasmid

   #159642

   Purpose

   Contains integrin alpha v residues 1–960 with the M400C mutation

   Depositor

   Timothy Springer

   Article

   Wang et al Proc Natl Acad Sci U S A. 2017 May 23;11

   Insert

   Integrin alpha v ectodomain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M400C

   Promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. optGluCl beta Y182F ECFP

   Plasmid

   #15105

   Depositor

   Henry Lester

   Article

   Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

   Insert

   GluCl beta (glc-2 Nematode)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA, Y182F

   Promoter

   Available Since

   June 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. mGarnet2-RITA

   Plasmid

   #104314

   Purpose

   codes for a fusion of fluorescent protein mGarnet2 and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

   Depositor

   Ulrich Nienhaus

   Article

   Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

   Insert

   mGarnet2-RITA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB2AR-miRFP670nano3

    Plasmid

    #184671

    Purpose

    Beta-2-adrenergic receptor internally tagged with miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    B2AR-miRFP670nano3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3-ySet2

    Plasmid

    #51698

    Purpose

    Expresses full length yeast Set 2 gene in a mamalian expression vector

    Depositor

    Tom Misteli

    Article

    Luco et al Science. 2010 Feb 19;327(5968):996-1000.

    Insert

    yeast Set2 (SET2 Budding Yeast)
    

    Use

    Tags

    C-terminal FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pBBI

    Plasmid

    #65795

    Purpose

    Vector for iCre mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al Transgenic Res. 2013 Aug;22(4):873-5. do

    Insert

    iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus rGBD

    Plasmid

    #99015

    Purpose

    Used as a RhoA-GTP sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    rGBD (Rtkn Mouse)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-Mirk (YF)

    Plasmid

    #17845

    Depositor

    Eileen Friedman

    Article

    Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

    Insert

    Mirk (DYRK1B Human)
    

    Use

    Tags

    6xHis and Anti-XPress

    Expression

    Mammalian

    Mutation

    Y271/273F

    Promoter

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

15. Cenp-B INCENP mCherry

    Plasmid

    #45235

    Purpose

    Target INCENP to centromeres via full length CENP-B

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B, INCENP-Δcen, mCherry
    

    Use

    Tags

    full length CENP-B and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4xHRE_minSV40-sfGFP-CMV_dsRed

    Plasmid

    #117400

    Purpose

    sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1 (+) Tacδ

    Plasmid

    #81177

    Purpose

    Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    Tacδ
    

    Use

    Tags

    KLGFFKR

    Expression

    Mammalian

    Mutation

    CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-ELYS-emerin-EGFP-polyA

    Plasmid

    #59745

    Purpose

    Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    ELYS-emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAL190

    Plasmid

    #22279

    Purpose

    Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    TimDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-RNF126 C231A

    Plasmid

    #138648

    Purpose

    Mammalian expression of full-length2 HA-tagged RNF126 with an inactivating mutation in the RING domain (C231A)

    Depositor

    Jane McGlade

    Article

    Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

    Insert

    RNF126 (RNF126 Mouse)
    

    Use

    Tags

    HA-HA

    Expression

    Mammalian

    Mutation

    C231A

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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