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  6. pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199367
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    TEV-SUMOEu-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199367-plasmid-map-sequence-id-389911

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  7. pTS116 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid
    #199369
    Purpose
    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.
    Depositor
    Rebecca Voorhees
    Article
    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    TEV-SUMOstar-ALFA-P2A-TagBFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    199369-plasmid-map-sequence-id-389901

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  15. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Venkat Gopalan
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Use
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231487-plasmid-map-sequence-id-460673

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  16. pAIP1

    Plasmid
    #233685
    Purpose
    Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution.
    Depositor
    Christopher Bahl
    Insert
    Ulp1-like SUMO protease
    Use
    Tags
    CAHS-107838 and HisTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 4, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233685-plasmid-map-sequence-id-463803

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  17. pCIOX

    Plasmid
    #51300
    Purpose
    E.coli expression vector for His8-SUMO tagged recombinant protein
    Depositor
    Andrea Mattevi
    Type
    Empty backbone
    Use
    Tags
    His8 + SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    51300-plasmid-map-sequence-id-78111

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  18. PAS_RvSAHS6

    Plasmid
    #215647
    Purpose
    Expresses SUMO-tagged RvSAHS6 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS6
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215647-plasmid-map-sequence-id-426094

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  19. PAS_RvSAHS1

    Plasmid
    #215645
    Purpose
    Expresses SUMO-tagged RvSAHS1 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS1
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215645-plasmid-map-sequence-id-426092

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  20. PAS_HeSAHS4

    Plasmid
    #215648
    Purpose
    Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS4
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215648-plasmid-map-sequence-id-426093

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  21. PAS_RvSAHS4

    Plasmid
    #215646
    Purpose
    Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli
    Depositor
    Pamela Silver
    Insert
    SAHS4
    Use
    Tags
    SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215646-plasmid-map-sequence-id-426091

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  22. pET28a-UBC9

    Plasmid
    #228949
    Purpose
    express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228949-plasmid-map-sequence-id-472392

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  23. pET28a-UBC9-K14R-A129K

    Plasmid
    #228948
    Purpose
    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    changed Lysine 14 to Arginine, changed Alanine 12…
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228948-plasmid-map-sequence-id-472389

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  24. pET28a-RANGAP1-CTD

    Plasmid
    #228947
    Purpose
    express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized
    Depositor
    Marcin Suskiewicz
    Insert
    RANGAP1-CTD (RANGAP1 Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    contains aa 418-587 only
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228947-plasmid-map-sequence-id-472386

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  25. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Use
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  26. pAIP4

    Plasmid
    #233686
    Purpose
    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.
    Depositor
    Christopher Bahl
    Insert
    Smt3 (SMT3 )
    Use
    Tags
    HisTag
    Expression
    Mutation
    Promoter
    T7
    Available since
    April 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233686-plasmid-map-sequence-id-468017

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  27. PML-3/pSG5

    Plasmid
    #50939
    Purpose
    expression of PML with Sumo sites destroyed
    Depositor
    Pier Pandolfi
    Article
    Zhong et al Blood. 2000 May 1;95(9):2748-52.
    Insert
    PML-3 (PML Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K65R, K160R, K490R (Sumo modification sites)
    Promoter
    SV40
    Available since
    Jan. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 451 - 480 of 1225 results