Skip to main content
Addgene
Showing: 751 - 800 of 1206 results

  22. pCMV-3UTR

    Plasmid
    #202550
    Purpose
    Ready for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation media
    Depositor
    Rostislav Skrabana
    Article
    Meskova et al Antibodies (Basel). 2023 Aug 4;12(3):51.
    Type
    Empty backbone
    Use
    Tags
    ATG initiation codon followed by an IGKV3 signal …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    202550-plasmid-map-sequence-id-405353

    Enlarge

  25. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Use
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

    Enlarge

  39. pCOTS-pyl-GFP(35TAG)

    Plasmid
    #92047
    Purpose
    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Replicative expression plasmid for cyanobacteria …
    Tags
    Histag
    Expression
    Mutation
    Tyrosine in position 35 of the GFP was mutated f…
    Promoter
    LeuP (native S. elongatus promoter), PpsbII, and …
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92047-plasmid-map-sequence-id-194905

    Enlarge

  42. pX020

    Plasmid
    #167146
    Purpose
    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal caffeoylpiruvate hydrolase, nnCPH
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167146-plasmid-map-sequence-id-335229

    Enlarge

  46. pN094

    Plasmid
    #167155
    Purpose
    MoClo-compatible Level1 (position 6) vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-npgA-ocsT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167155-plasmid-map-sequence-id-335276

    Enlarge

  47. pAAV2-CAGIG-H2B-V5-mScarlet

    Plasmid
    #191093
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    H2B and mScarlet
    Use
    AAV
    Tags
    H2B-V5-mScarlet
    Expression
    Mammalian
    Mutation
    The fusion protein was optimized to the Human cod…
    Promoter
    CMV and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191093-plasmid-map-sequence-id-380126

    Enlarge

  48. TFORF2582

    Plasmid
    #142436
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NR1D1 (NR1D1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142436-plasmid-map-sequence-id-397216

    Enlarge

  50. pSHUT4-eYFP

    Plasmid
    #133094
    Purpose
    Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI
    Depositor
    Jens Laurids Sørensen
    Article
    Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25
    Insert
    enhanced yellow flourescent protein
    Use
    Fungal expression
    Tags
    Expression
    Mutation
    Promoter
    TEF-1α
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133094-plasmid-map-sequence-id-266874

    Enlarge

Showing: 751 - 800 of 1206 results