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pcDNA3 Tat HA (P#29)

Plasmid

#14654

Depositor

Matija Peterlin

Article

Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

Insert

Tat (tat HIV)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pRPR1_gRNA_handle_RPR1t

Plasmid

#49014

Purpose

gRNA expression empty vector (yeast)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

pRPR1

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Article

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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RNA-binding protein pooled CRISPR knockout library

Pooled Library

#141438

Purpose

Knockout library targeting RNA-binding proteins with 10 guide RNAs per target.

Depositor

Eugene Yeo

Article

Wheeler et al Nat Methods. 2020 May 11. pii: 10.1038/s

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only
pMSP1

Plasmid

#20060

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of deletion mutant (1-43) of human…

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pT3TS-Tol2

Plasmid

#31831

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Cloning vector

Promoter

Lac

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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Stat3 S727A pRc/CMV

Plasmid

#8708

Depositor

Jim Darnell

Article

Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

Insert

Stat3 S727A (Stat3 Mouse)

Expression

Mammalian

Mutation

S727A (exhibits o serine phosphorylation either c…

Available Since

Aug. 11, 2005

Availability

Academic Institutions and Nonprofits only

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Human Beta-catenin GFP

Plasmid

#71367

Purpose

mammalian expression of human Beta-catenin GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

Beta-catenin (CTNNB1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CD68 promoter/enhancer

Plasmid

#34837

Depositor

Peter Murray

Article

Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

Insert

CD68 promoter/enhancer -680/+140 (CD68 Human)

Expression

Mammalian

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

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EGFP-Bak

Plasmid

#32564

Depositor

Richard Youle

Article

Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

Insert

human Bak (BAK1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

Plasmid

#154473

Purpose

Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

SFFV

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hSox2

Plasmid

#19779

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Sox2 (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV4-BlaM-Vpr

Plasmid

#21950

Depositor

Warner Greene

Article

Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

Insert

BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
p3868 HA-E6-AP

Plasmid

#8648

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Article

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hOct4

Plasmid

#19778

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Oct4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has Service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pARE-lux

Plasmid

#11768

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

ARE

Use

Luciferase

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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3544 pMIG Bcl-2

Plasmid

#8793

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-2 (BCL2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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