1. Myr-(iSH2-p85)-p110alpha-Myc

   Plasmid

   #1410

   Depositor

   Lewis Cantley

   Insert

   PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. 1068 pGL3 FasL promoter (no SV40 prom)

   Plasmid

   #9029

   Depositor

   William Sellers

   Article

   Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

   Insert

   Fas ligand promoter (FASLG Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Removed the SV40 promoter from pGL3

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pAR69_STRI_m(KS_MAT)_H8_pET22b

   Plasmid

   #122849

   Purpose

   Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   m(KS_MAT) (Fasn Mouse)
   

   Use

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLCMV:ECFP(loxP)(FRT)HNF1B

   Plasmid

   #31439

   Depositor

   Gerhart Ryffel

   Article

   Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Cre/Lox; Flp/frt

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLCMV:ECFP(loxP)(FRT)HNF1B_P328L329del

   Plasmid

   #30528

   Depositor

   Gerhart Ryffel

   Article

   Roose et al Transgenic Res. 2009 Aug . 18(4):595-605

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Cre/Lox; Flp/frt

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P328L329delCCTCT

   Promoter

   Available since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLJM5-I560S-mDPYD

   Plasmid

   #61615

   Purpose

   The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

   Depositor

   David Sabatini

   Article

   Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

   Insert

   Dihydropyrimidine dehydrogenase (Dpyd Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Changed Isoleucine at position 560 to Serine

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLCMV:ECFP(loxP)(FRT)HNF1B_A263ins

   Plasmid

   #31440

   Depositor

   Gerhart Ryffel

   Article

   Sauert et al PLoS One. 2012;7(3):e33522. Epub 2012 Ma

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Cre/Lox; Flp/frt

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   A263insGG

   Promoter

   Available since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF1214

   Plasmid

   #142756

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXH1 (FOXH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiCRISPR v2-sgCYFIP1-1

   Plasmid

   #210137

   Purpose

   knock out CYFIP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiCRISPR v2-sgCYFIP1-2

   Plasmid

   #210138

   Purpose

   knock out CYFIP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. IGFN1 gRNA (BRDN0001147410)

    Plasmid

    #76823

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IGFN1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IGFN1 (IGFN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. IGFN1 gRNA (BRDN0001144979)

    Plasmid

    #76824

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IGFN1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IGFN1 (IGFN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. IGFN1 gRNA (BRDN0001146137)

    Plasmid

    #76825

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IGFN1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IGFN1 (IGFN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IGFN1 gRNA (BRDN0001147077)

    Plasmid

    #76826

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IGFN1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IGFN1 (IGFN1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FAS_pLX307

    Plasmid

    #98334

    Purpose

    Lentiviral expression of FAS

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    FAS (FAS Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-sgFat1#1/Cre

    Plasmid

    #173633

    Purpose

    Expresses a Fat1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Fat1 (Fat1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(FOXH1_5-2)-PGKpuroBFP-W

    Plasmid

    #211962

    Purpose

    Express gRNA against FOXH1 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting FOXH1 (FOXH1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(FOXH1_5-4)-PGKpuroBFP-W

    Plasmid

    #211963

    Purpose

    Express gRNA against FOXH1 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting FOXH1 (FOXH1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-sgFat1#2/Cre

    Plasmid

    #173634

    Purpose

    Expresses a Fat1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Fat1 (Fat1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF1686

    Plasmid

    #143966

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIG-835_NY-ESO-1_TCR_FAS/MyD88

    Plasmid

    #207498

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/MyD88, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge