We narrowed to 7,975 results for: AMPH

1. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

   Plasmid

   #225649

   Purpose

   tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

   Depositor

   Andre Sanches Ribeiro

   Article

   Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

   Insert

   tetR/tetA, araBAD and LacO3O1 in tandem conformation
   

   Tags

   mCherry

   Expression

   Bacterial

   Promoter

   tetA, araBAD and LacO3O1 promoters

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNatA (pACYCduet-naa10-naa15)

   Plasmid

   #72928

   Purpose

   Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   naa10 (naa10 Fission Yeast, Synthetic)
   naa15 (naa15 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Codon optomised for E.coli expression

   Promoter

   T7

   Available Since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-HA-Tuba

   Plasmid

   #22214

   Depositor

   Pietro De Camilli

   Article

   Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

   Insert

   Tuba (DNMBP Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-Delta(Pro-MP)ADAM10-HA

   Plasmid

   #65107

   Purpose

   Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   Delta(Pro-MP)ADAM10 (ADAM10 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted AA 19-455

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pMDC32B-AtTAS1c-D2-B/c

   Plasmid

   #137884

   Purpose

   Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]

   Depositor

   Alberto Carbonell

   Article

   Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

   Insert

   AtTAS1c-D2-B/c
   

   Expression

   Plant

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   2x35S

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-AtTAS1c-D2-B/c

   Plasmid

   #137883

   Purpose

   Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]

   Depositor

   Alberto Carbonell

   Article

   Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

   Insert

   AtTAS1c-D2-B/c
   

   Use

   Gateway-compatible entry clone

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. YFP-synDAT-S/A

   Plasmid

   #48792

   Purpose

   Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro

   Depositor

   Jonathan Javitch

   Article

   Fog et al Neuron. 2006 Aug 17;51(4):417-29.

   Insert

   YFP-synhDAT-S/A (SLC6A3 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. YFP-synDAT-S/D

   Plasmid

   #48793

   Purpose

   Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro

   Depositor

   Jonathan Javitch

   Article

   Fog et al Neuron. 2006 Aug 17;51(4):417-29.

   Insert

   YFP-synhDAT-S/D (SLC6A3 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. PL-5LTR-GW-A

   Plasmid

   #138480

   Purpose

   For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid

    #171089

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Mutation

    D10A, H840A

    Promoter

    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Thy1.1-G5

    Plasmid

    #234839

    Purpose

    pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

    Depositor

    Gabriel Victora

    Article

    Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

    Insert

    Thy1.1 (Thy1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-P2A-5xGly

    Expression

    Mammalian

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid

    #171090

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

    Mutation

    D9A, H599A

    Promoter

    TUB1 (Toxoplasma gondii), transcriptionally linke…

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBGE3.0

    Plasmid

    #165988

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    neomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGE2.0

    Plasmid

    #165984

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    tetracycline resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBGE1.0

    Plasmid

    #165986

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGE1.0

    Plasmid

    #165983

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Spec resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    spectinomycin/streptomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBGE2.0

    Plasmid

    #165987

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    tetracycline resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAGE3.0

    Plasmid

    #165985

    Purpose

    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Nm/Kan resistance.

    Depositor

    Bogumil Karas

    Article

    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

    Inserts

    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    neomycin resistance
    

    Use

    Synthetic Biology; Bacterial and yeast cloning; c…

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid

    #137885

    Purpose

    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Inserts

    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    

    Expression

    Plant

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPos-Ma-CmR-TAG111

    Plasmid

    #197571

    Purpose

    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    

    Expression

    Bacterial

    Mutation

    111TAG and 8TAG/112TAG

    Promoter

    AraC, T7, cat promoter (constitutive), and lpp (c…

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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