We narrowed to 4,676 results for: EFS

1. CROPseq-EFS-HypaSpCas9-P2A-puro

   Plasmid

   #106965

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

   Plasmid

   #138152

   Purpose

   Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   eGFP-MCS
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROPseq-EFS-HypaSpCas9-P2A-EGFP

   Plasmid

   #106964

   Purpose

   single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

   Depositor

   Alex Hewitt

   Insert

   HypaSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOT_2 - lenti-EFS-tNGFR-2A-puro

   Plasmid

   #181971

   Purpose

   Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   tNGFR (NGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   truncation: aa 11-276

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

   Plasmid

   #192148

   Purpose

   T cell CRISPR AAV vector

   Depositor

   Sidi Chen

   Article

   Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

   Plasmid

   #154003

   Purpose

   Vector expressed CasRx and sgRNA_LACZ for AAV package

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   CasRx
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLY100-RetroEFS-HER2-28BBz-WPRE

   Plasmid

   #192201

   Purpose

   Retro-HER2CAR

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-HER2CAR (ERBB2 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EFS-PEmax-P2A-Puro

   Plasmid

   #228890

   Purpose

   Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Inserts

   PEmax
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLY093-EFS-BCMA-Blast-WPRE

   Plasmid

   #192194

   Purpose

   Lenti-BCMA-OE-Blast

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-BCMA-OE-Blast (TNFRSF17 Synthetic)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-EFS-foldon-GFP- PCP

    Plasmid

    #194503

    Purpose

    Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Insert

    foldon-GFP-PCP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α core promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-EFS-EGFP-T2A-Bsd

    Plasmid

    #193198

    Purpose

    Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-EFS-LSL-nlsGFP-shRNA

    Plasmid

    #175584

    Purpose

    Conditional express shRNA with control of LoxP

    Depositor

    Christian Wolfrum

    Article

    Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EFS-CjCas9-eGFP-HIF1a

    Plasmid

    #137929

    Purpose

    Expression of cjCas9 with Hif1a targeting sgRNA

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    SV40NLS-HA-T2A-EGFP

    Expression

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

    Plasmid

    #172436

    Purpose

    Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    Cre
    mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLY094-EFS-CD22-Blast-WPRE

    Plasmid

    #192195

    Purpose

    Lenti-CD22-OE-Blast

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-CD22-OE-Blast (CD22 Synthetic)
    

    Use

    Lentiviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. hU6-pegRNA-EFS-mScarlet Template

    Plasmid

    #196037

    Purpose

    The template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and EFS

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJEP304-pAAV-EFS-dSaCas9-VP64-pA

    Plasmid

    #113679

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-EFS-PCP-3XNeon-nls

    Plasmid

    #75386

    Purpose

    PCP-3XNeon-nls

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    3XmNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

    Plasmid

    #78607

    Purpose

    A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

    Depositor

    Amy Wagers

    Article

    Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

    Insert

    EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
    

    Use

    AAV and CRISPR

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short; U6

    Available since

    Sept. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-EFS-dSa-VPR mini.

    Plasmid

    #99686

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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