We narrowed to 584 results for: LC3

1. Halo-LC3B HRD

   Plasmid

   #207539

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag with internal PuroR cassette flanked by human LC3B locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAsh-MCL-LC3B

   Plasmid

   #204573

   Purpose

   Expresses Ash-tagged LC3B in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   delta121-125aa

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGK EGFP-LC3B

   Plasmid

   #200427

   Purpose

   Mammalian expression of fluorescently-tagged marker for autophagic vesicles

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TalO8-GLC3-CFP

   Plasmid

   #178307

   Purpose

   CoTrIP plasmid with GLC3 promoter driving CFP's expression

   Depositor

   Brian Zid

   Article

   Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

   Insert

   Glc3pr-CFP-tAdh1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GLC3 and NA

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP.N3-myc-SLC35A2

   Plasmid

   #186285

   Purpose

   transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

   Insert

   SLC35A2 (SLC35A2 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pStA0::SPLc3-RBSc110

   Plasmid

   #171893

   Purpose

   Promoter SPLc3 and RBSc110 in pStA0

   Depositor

   John Heap

   Article

   Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

   Insert

   Promoter SPLc3 and RBSc110
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pStA0::SPLc3-RBSc249

   Plasmid

   #171903

   Purpose

   Promoter SPLc3 and RBSc249 in pStA0

   Depositor

   John Heap

   Article

   Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

   Insert

   Promoter SPLc3 and RBSc249
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pStA0::SPLc3-RBSc21

   Plasmid

   #171884

   Purpose

   Promoter SPLc3 and RBSc21 in pStA0

   Depositor

   John Heap

   Article

   Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

   Insert

   Promoter SPLc3 and RBSc21
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SLC35B2-p2A-E2Crimson

   Plasmid

   #83941

   Purpose

   Lentiviral vector expressing SLC35B2-p2A-E2Crimson

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Use

   Lentiviral

   Tags

   p2A-E2Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223 LC3B2 no-stop

    Plasmid

    #123207

    Purpose

    Gateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Stop codon removed

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUW mCherry-GFP-LC3

    Plasmid

    #110060

    Purpose

    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

    Depositor

    Anne Brunet

    Article

    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

    Insert

    mCherry-GFP-LC3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IP-GFP-LC3-RFP

    Plasmid

    #84573

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-No-HaloTag7-mGFP-LC3

    Plasmid

    #184901

    Purpose

    Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs GFP-LC3-RFP

    Plasmid

    #117413

    Purpose

    Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST-CMV GFP-LC3B WT

    Plasmid

    #123114

    Purpose

    Expresses EGFP-LC3B wild-type in mammalian cells. Fluorescent reporter for autophagosomes.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-myc-LC3 (Atg8)

    Plasmid

    #24919

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    LC3 (MAP1LC3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV 3xFLAG-LC3B G120

    Plasmid

    #123094

    Purpose

    Expresses 3xFLAG-LC3B G120 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV 3xFLAG-LC3B G120A

    Plasmid

    #123093

    Purpose

    Expresses 3xFLAG-LC3B G120A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. [pSK104] pET28 SLC38A9N-His

    Plasmid

    #136142

    Purpose

    bacterial expression of SLC38A9 N term fragment (1-119)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    SLC38A9 N term fragment (1-119) (SLC38A9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-FLAG-APEX2-MAP1LC3B

    Plasmid

    #175753

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge