We narrowed to 11,114 results for: Lif

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pcDNA3-LifeAct-FRB-Venus-173-N

Plasmid

#87616

Purpose

FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-Venus173-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

Plasmid

#99229

Purpose

Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos2

Tags

Lifeact

Expression

Mammalian

Mutation

A69T for primed conversion applications

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-FRB-DMVF-181-N

Plasmid

#87610

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-DMVF181-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

Plasmid

#171436

Purpose

Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-Lifeact

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

Plasmid

#171435

Purpose

Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

Lifeact-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-Lifeact- mScarlet-HA (JDW 1247)

Plasmid

#224538

Purpose

A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mScarlet-HA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Lifeact-HaloTag7

Plasmid

#187076

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 on actin filaments

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_Lifeact-HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-FRB-Venus-173-N

Plasmid

#87616

Purpose

FRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-Venus173-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2020

Availability

Academic Institutions and Nonprofits only

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FHL-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129628

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

FHL-cpmTq2-Calcium-lifetime-sensor

Tags

6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial and Mammalian

Promoter

CMV,&Rhamnose

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lck-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129627

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

Lck-cpmTq2-Calcium-lifetime-sensor

Tags

Lck

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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3xnls-cpmTq2-Calcium-lifetime-sensor

Plasmid

#129626

Purpose

Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

Depositor

Dorus Gadella

Article

van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

Insert

3xnls-cpmTq2-Calcium-lifetime-sensor

Tags

3xNLS

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

Plasmid

#99229

Purpose

Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos2

Tags

Lifeact

Expression

Mammalian

Mutation

A69T for primed conversion applications

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-syn-LifeAct-jGCaMP8m-WPRE

Plasmid

#186039

Purpose

AAV-mediated expression of LifeAct-jGCaMP8m under the synapsin promoter

Depositor

Loren Looger

Insert

LifeAct-jGCaMP8m

Use

AAV

Tags

LifeAct-6xHis

Expression

Mammalian

Promoter

synapsin

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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p35S::mCh-HA-P2A-lifeact-mCh

Plasmid

#135234

Purpose

constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

mCh-HA-P2A-lifeact-mCh

Tags

HA, lifeact, and p2a

Expression

Plant

Promoter

35S

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-lifeact-mNeongreen-ORF-1

Plasmid

#241204

Purpose

Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

lifeact-mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-lifeact-mNeongreen-ORF-2

Plasmid

#241205

Purpose

Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

lifeact-mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-lifeact-mNeongreen-ORF-3

Plasmid

#241206

Purpose

Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

lifeact-mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-lifeact-mNeongreen-ORF-1

Plasmid

#241172

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-lifeact-mNeongreen-ORF-2

Plasmid

#241173

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-lifeact-mNeongreen-ORF-3

Plasmid

#241174

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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