Addgene

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KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

Plasmid

#232936

Purpose

Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

T2A-eGFP-SV40pA with Podxl homology arms

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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Pxyl-rsaA

Plasmid

#207250

Purpose

Integrative plasmid for expressing rsaA gene (incl. its promoter) in Caulobacter crescentus at native Pxyl promoter region

Depositor

Suliana Manley

Article

Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:

Insert

rsaA (promoter to codon sequence)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHB1

Plasmid

#204668

Purpose

pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

Depositor

Mark Mandel

Article

Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

Insert

FRT-erm-FRT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-blastR-p2a-v5-dL5

Plasmid

#161980

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

blastR-p2a-v5-dL5-GSlinker

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-v5-dL5-p2a-blastR

Plasmid

#161981

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GSlinker-V5-dL5-P2A-blastR

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr1508

Plasmid

#136746

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1508

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr1508 (Or4e1 Mouse)

Use

In situ probe

Tags

Expression

Mutation

ISH Probe insert

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr156

Plasmid

#139486

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH)

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

ISH probe for Olfr156 - UTR (Olfr156 Mouse)

Use

Ish probe

Tags

Expression

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-pT7-sfGFP_RED20

Plasmid

#135173

Purpose

Expresses sfGFP recoded in the RED20 genetic code off the T7 promoter

Depositor

Drew Endy

Article

Calles et al Nucleic Acids Res. 2019 Sep 12. pii: 556

Insert

sfGFP_RED20

Use

Synthetic Biology

Tags

Expression

Mutation

Recoded ORF of sfGFP to only use one codon per am…

Promoter

taatacgactcactata

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Stmn2

Plasmid

#74397

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Stmn2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

stathmin-like 2 (Stmn2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBS xenopus Endodermin (pBS-eddL6)

Plasmid

#24968

Depositor

Edward De Robertis

Article

Sasai et al EMBO J. 1996 Sep 2. 15(17):4547-55.

Insert

Endodermin (a2m Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
xfz8 probe/pBS

Plasmid

#16323

Depositor

Peter Klein

Article

Deardorff et al Development. 1998 Jul . 125(14):2687-700

Insert

frizzled-8 (fzd8.S Frog)

Use

Tags

Expression

Bacterial

Mutation

Base pairs 1159-3240 of xfz8 cDNA

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCMVtrunc Affimer6-sfCherry2ΔN12

Plasmid

#231551

Purpose

Mammalian expression of Affimer6 fused to N-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Affimer6

Use

Tags

sfCherry2

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc Affimer6-msfGFPΔN12

Plasmid

#231550

Purpose

Mammalian expression of Affimer6 fused to N-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

Insert

Affimer6

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Thomas Pollard, Julien Berro

Article

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTuLys2CMR2

Plasmid

#53544

Purpose

Inhibition module. Constructed from the parent plasmids pLuxRI, pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 lysozyme by inducing AHL and LuxR. Chloramphenicol resistance

Depositor

Lingchong You

Article

Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

Insert

pLux

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRM006

Plasmid

#98922

Purpose

Express Histag-SUMO-FtsZ for purification in E coli

Depositor

Jie Xiao

Article

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsZ

Use

Tags

Histag-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pPGxRF3

Plasmid

#138384

Purpose

T2A-GAL4 knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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TANGO1S-mScarlet-i

Plasmid

#165461

Purpose

Encodes TANGO1S tagged with mScarlet-i

Depositor

David Stephens

Article

McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

Insert

TANGO1S

Use

Tags

mScarlet-i

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1

Plasmid

#12209

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV6M-PAK1 T423E

Plasmid

#12208

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Activated Pak1: T423E

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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